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Cloning of a gene expressed during appressorium formation by Colletotrichum gloeosporiodes and a marked decrease in virulence by disruption of this gene. Plant Cell 7:183–193

By Cheng-shine Hwang and Moshe A. Flaishman

Abstract

Appressorium formation in germinating Colletotrichum gloeosporioides is induced by the surface wax of its host. One of the genes expressed uniquely in C. gloeosporioides during appressorium formation induced by the host signal has been designated cap20, and this gene and its cDNA were cloned and sequenced. Nucleotide sequences of both revealed an open reading frame that could encode a 183-amino acid polypeptide that did not have significant homology with any known proteins. Reverse transcriptase-polymerase chain reaction detected cap2O gene transcripts at the infection front on the surface and within tomato fruits infected by C. gloeosporioides. Gene-disrupted mutants incapable of expressing cap20showed a drastically decreased virulence on awocado and tomato fruits. These results suggest that Cap20 plays a significant role in the infection of the host

Year: 1995
OAI identifier: oai:CiteSeerX.psu:10.1.1.319.4639
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