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Modulation of Aqueous Humor Outflow by Ionic Mechanisms Involved in Trabecular Meshwork Cell Volume Regulation

By David Soto, Núria Comes, Elisa Ferrer, Miguel Morales, Artur Escalada, Jordi Palés, Carles Solsona, Arcadi Gual and Xavier Gasull


and their interactions with extracellular matrix determine outflow facility. Because cell volume seems essential to TM function, this study was conducted to investigate further the ionic channels and receptors involved in regulatory volume decrease and their roles in modulating outflow facility. METHODS. Primary cultures of bovine TM cells were used. K � and Cl � currents were studied with whole-cell patch clamping. Swelling was induced by hypotonic shock. [Ca 2 � ] i was measured in TM cells loaded with fura-2. Bovine anterior segments were perfused at constant pressure to measure outflow facility. RESULTS. Hypotonic media activated both the high-conductance Ca 2 �-activated K � channel (BK Ca) and swelling-activated Cl � channel (Cl swell) currents and induced release of adenosine 5�-triphosphate (ATP) from TM cells. ATP activated P2Y 2 receptors with the following profile: ATP � uridine 5�-triphosphat

Year: 2013
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