Location of Repository

Immunohistochemical Localization of Transforming Growth Factor-/?1,-/?2, and-03 Latency-Associated Peptide in Human Cornea

By Kohji Hashimoto and Shuji Yamamoto

Abstract

Purpose. To study spatial distribution of TGF-/3 isoforms (TGF-/31,-/32, and-/33) in the human cornea and to elucidate their biologic roles in corneal maintenance. Methods. Frozen sections obtained from eight human autopsy eyes were placed on gelatincoated slides. After blocking of nonspecific binding sites, the slides were incubated with rabbit polyclonal antibody to the latency-associated peptide (LAP) region of human TGF-01, TGF-02, and TGF-/33 precursors, followed by the incubation with biotinylated swine anti-rabbit IgG. Subsequently, a streptavidin-labeled alkaline phosphatase technique was used. Results. In the corneal region, /31-LAP antibody did not stain either epithelium or stroma, /32-LAP antibody stained all epithelial cell layers and the corneal stroma, and 03-LAP antibody stained the subepithelial region alone. The staining pattern in the limbal region was almost the same as in the corneal region, except in the limbal stroma, which was stained with /31-LAP antibody in three of eight samples. In the trabecular meshwork, all samples showed clear staining with /32-LAP antibody, whereas /31-LAP and /32-LAP antibody stained faintly in five of eight and four of eight samples, respectively. Conclusion. /82-LAP was found in the corneal epithelium and stroma and /33-LAP in the subepithelia

Year: 2013
OAI identifier: oai:CiteSeerX.psu:10.1.1.319.2772
Provided by: CiteSeerX
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://citeseerx.ist.psu.edu/v... (external link)
  • http://www.iovs.org/content/35... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.