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Interleukin 1 2haivates Two Forms of p54a Mitogen-activated Protein K inase in Rabbit Liver

By Michael Kracht, Oanh Truong, Nick F. Totty, Masahiro Shiroo and Jeremy Saklatvala


We have identified in rabbits two hepatic forms of T669 peptide kinases that are very strongly activated after systemic injection with the inflammatory cytokiue interleukin 1 (ID1). The T669 peptide contains a major phosphoryhtion site of the epidermal growth factor receptor, threonine 699 and is a substrate for mitogen-activated protein (MAP) kinases. The kinases were purified to homogeneity and corresponded to 50- and 55-kD proteins on sodium dodecyl sulfate-polyaerylamide gel electrophoresis. Amino add sequencing of 12 tryptic peptides of both kinases identified them as p54 MAP kinase or. This kinase belongs to the novel family of stress-activated protein kinag's. This is the first evidence of IL-1 activating a spedfic protein kinase in vivo. Downloaded from I L-1 is a major inflammatory hormone. It is produced at sites of injury or infection where it causes leukocyte accumulation, cytokine production, and resorption of connective tissue matrix. If it enters the circulation it induces the acute phase response and fever (1)

Year: 1994
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