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Assembly and disassembly of the Golgi complex: two processes arranged in a cis-trans direction

By Jose Alcalde, Pedro Bonay, Ana Roa, Senen Vilaro and Ignacio V S


Abstract. We have studied the disassembly and assembly of two morphologically and functionally distinct parts of the Golgi complex, the cis/middle and trans cisterna/Trans network compartments. For this purpose we have followed the redistribution of three cis/middle- (GMP-,, GMP- 2, MG 160) and two trans- (GMP,-, and GMP,- 2) Golgi membrane proteins during and after treatment of normal rat kidney (NRK) cells with brefeldin A (BFA). BFA induced complete disassembly of the cis/middle- and trans-Golgi complex and translocation of GMP, and GMP, to the ER. Cells treated for short times (3 min) with BFA showed extensive disorganization of both cis/middle- and trans-Golgi complexes. However, complete disorganization of the trans part required much longer incubations with the drug. Upon removal of BFA the Golgi complex was reassembled by a process consisting of Golgi complex plays an important role in the post- THE translational modifications and sorting of proteins transported from the ER, recycling of receptors involved in endocytosis and transport ofproteins to organelles, and in the resorting of ligand-receptor complexes internalized by transcytosis (Palade, 1975; Farquhar, 1985; Pfeffer and Rothman, 1987). The organelle is constituted by two morphologically and functionally distinct parts: a stack of cisternae (Morr6 and Ovtracht, 1977; Farquhar and Palade 1981), and a network of tubules called the trans networ

Year: 1992
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