Article thumbnail

Towards early-warning gene signature of <i>Chlamydomonas reinhardtii</i> exposed to Hg-containing complex media

By Rebecca Beauvais-Flueck, Vera Slaveykova, Skyllberg Ulf and Claudia Cosio

Abstract

The potential of using gene expression signature as a biomarker of toxicants exposure was explored in the microalga Chlamydomonas reinhardtii exposed 2 h to mercury (Hg) as inorganic mercury (IHg) and methyl mercury (MeHg) in presence of copper (Cu) and Suwannee River Humic Acid (SRHA). Total cellular Hg (THg = IHg + MeHg) decreased in presence of SRHA for 0.7 nM IHg and 0.4 nM MeHg, but increased for 70 nM IHg exposure. In mixtures of IHg + MeHg and (IHg or MeHg) + Cu, SRHA decreased THg uptake, except for 0.7 nM IHg + 0.4 nM MeHg which was unchanged (p-value>0.05). In the absence of SRHA, 0.5 μM Cu strongly decreased intracellular THg concentration for 70 nM IHg, while it had no effect for 0.7 nM IHg and 0.4 nM MeHg. The expression of single transcripts was not correlated with measured THg uptake, but a subset of 60 transcripts showed signatures specific to the exposed metal(s) and was congruent with exposure concentration. Notably, the range of fold change values of this subset correlated with THg bioaccumulation with a two-slope pattern in line with [THg]intra/[THg]med ratios. Gene expression signature seems a promising approach to complement chemical analyses to assess bioavailability of toxicants in presence of other metals and organic matter

Topics: info:eu-repo/classification/ddc/550, info:eu-repo/classification/ddc/333.7-333.9, Copper, Dissolved organic matter, Microalgae, Uptake, Transcriptomics
Publisher: 'Elsevier BV'
Year: 2019
DOI identifier: 10.1016/j.aquatox.2019.105259
OAI identifier: oai:unige.ch:unige:122745
Provided by: Archive ouverte UNIGE
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://archive-ouverte.unige.... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.