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Printed in U.S.A. Copyright © 2002 by The Endocrine Society Mitogen-Activated Protein Kinase Activity in Cumulus Cells Is Essential for Gonadotropin-Induced Oocyte Meiotic Resumption and Cumulus Expansion in the Mouse

By You-qiang Su, Karen Wigglesworth, Frank L. Pendola, Marilyn J. O’brien and John J. Eppig


This study investigated the participation of MAPK in the resumption of meiosis [germinal vesicle breakdown (GVB)] in oocytes and cumulus expansion using oocyte-cumulus cell complexes (OCC) from Mos-null mice (Mos tm1Ev /Mos tm1Ev, hereafter Mos �/ �). MAPK activity was not detected in Mos �/ � oocytes whether they matured in vivo or in vitro, with or without gonadotropin stimulation. Therefore, there are no pathways independent of MOS that activate MAPK during gonadotropin-induced maturation. In contrast, MAPK activity was always detected coincident with GVB in Mos �/ � oocytes. Moreover, MAPK activity was detected in cumulus cells before gonadotropin-induced GVB in OCC regardless of genotype. A specific inhibitor (U0126) of MEK, a MAPKK required for MAPK activity, inhibited gonadotropin-induced GVB in OCC of both Mos �/ � and Mos �/ � mice. Activation of MAPK wa

Year: 2013
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