Figure 1. Electron micrographs of the bacterial symbionts and the gut pouch of a worker of the ant Tetraponera binghami. (a) Scanning electron micrograph showing the bacterial pouch (BP), Malpighian tubules (mt) and tracheal air supply. The intestine (INT) and midgut (MG) are also visible (scale bar, 100 µm). Reprinted from J. Billen & A. Buschinger 2001, with permission. (b) Detail (transmission electron micrograph (TEM)) of the bacterial contents of the pouch lumen (scale bar, 0.5 µm). (c) Overview (TEM) showing typical bacterial aggregations associated with the inner epithelium (right hand side of the figure) at the tip of the pouch. Cross-sections through tracheae (tr) are also indicated (scale bar, 10 µm). refinement. The resulting dataset included 1517 nucleotide sites. The sequences were aligned to a subset of closely related reference sequences (29 sequences found in GenBank), including the two closest relatives of each pouch symbiont, a number of close relatives with sequence similarities greater than 95% (without discriminating between nitrogen-fixing and other bacteria) and a number of sequences representing taxa with known ecological features and sister genera of Rhizobium. The photosynthetic filamentous bacterium Chloroflexus aggregan
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