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TILO MATERNA † AND UWE HARMENING Biocenter of the Johann-Wolfgang-Goethe-University,

By A Small-scale and Northern Hybridization Digoxigeninlabeled


We describe the use of PCR to generate sense and antisense template DNA for nonradioactive in vitro transcription reactions. The resulting digoxigenin-labeled RNA probes can be used in a small-scale Northern hybridization procedure that is performed in microcentrifuge tubes. This kind of Northern approach is especially useful when the expression of several genes is to be initially checked, for example, with a set of cDNA clones from a library screening. were designed (Ti1: 5'-CTT GGT CTG AC

Year: 2011
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