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Specific and Simple HPLC Assay of Ecofriendly Meloxicam in Pharmaceutical Formulations

By K. T. Mahmood, B. Khan, M. Ashraf and I. U Haq

Abstract

A simple, precise, specific,robust and rapid HPLC assay for the of measurement meloxicam in pharmaceutical formulations was developed and validated at Department of Pharmacy and Central Quality Assurance Laboratory, LCWU, Lahore. The solution of meloxicam standard was prepared in HPLC grade acetonitrile (5 μg.mL-1) and ultra sonicated for 15 minutes. One mL sample mixed well with one mL of HPLC grade water and filtered through 0.22 μm filter. Ten micro-litter sample was injected into HPLC system through an injector valve with a ten micro-litter sample loop. The mobile phase comprising of phosphate buffer(0.2 Normal) and acetonitrile (38:62, v/v) was pumped into Water 1525 Binary HPLC Pump 1525 at the rate 0.5mL/min Separation was achieved by using a reversed phase C18 column (Phenomenex, particle size 5 μm;4.6 mm ×150 mm) at retention time of 7.4 minutes. Oven temperature was set at 25 °C. The meloxicam was distinctly and specifically detected at 352nm by using Water 2487 dual absorbance detectors. The method was validated prior to the analysis of samples. The limit of detection and limit of quantification were.0.06 and 6 micrograms respectively. The sample from commercial pharmaceutical formulation were prepared in HPLC grade methanol in concentration of 5 μg.mL-1. The developed and validated method was successfully applied for assay of meloxicam in these formulations including injections, tablets and suspension. Keywords:Meloxicam; HPLC; C1.8 and Pharmaceutical Formulation

Year: 2011
OAI identifier: oai:CiteSeerX.psu:10.1.1.193.8694
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