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RESEARCH LETTER Transcriptomic microarrayanalysis of corrinoid responsive genes in Dehalococcoides ethenogenes strain195

By David R. Johnson, Audra Nemir, Gary L. Andersen, Stephen H. Zinder and Lisa Alvarez-cohen


Dehalococcoides ethenogenes strain 195 utilizes corrinoid-containing reductive dehalogenases to reduce the environmental pollutants tetrachloroethene and trichloroethene to ethene. Although corrinoids are essential for dehalogenation activity, strain 195 cannot biosynthesize corrinoids de novo. To improve our understanding of corrinoid physiology in this bacterium, whole-genome microarrays were applied to characterize the transcriptome during growth with excess and limiting concentrations of the corrinoid cyanocobalamin. Additional studies examined the effects of exposure to spent medium from a methanogenic chloroethene-dehalogenating enrichment culture (designated ANAS). Both excess cyanocobalamin and ANAS spent medium resulted in the downregulation of two genes (DET0125–0126) that are encoded downstream of a putative cobalamin riboswitch. In contrast, only ANAS spent medium resulted in the downregulation of three duplicated genes (DET0657–0659/DET0691–0693) encoded downstream of a second putative cobalamin riboswitch. These latter genes are predicted to b

Topics: Editor, Elizabeth Baggs Keywords Dehalococcoides, corrinoid, reductive halogenation
Year: 2009
OAI identifier: oai:CiteSeerX.psu:
Provided by: CiteSeerX
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