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By Atul Kumar, Manitha T. P, D. Jeya, Sundara Sharmila and K. Panneerselvam


Small lung carcinoma is an endemic disease throughout the world which occurs mainly by cigarette smoking. A mutagenous compound known as nitro benzene is formed in the body due to cigarette smoking which causes mutation in normal tissues of lung and hence they are changed into malignant tissues by over growth of the tissues. Cancer Genome Anatomy Project website developed by National Cancer Institute contains all the genomic data and different tools having different data sets. CGAP gives a chance to analyze all the wet lab data computationally and hence, CGAP was used to analyze the cause of small lung carcinoma. For this SAGE GENIE TOOL was used to get the SAGE tags as less expressed and over expressed in normal and malignant tissues. VIRTUAL NORTHERN BLOT TOOL was used to justify the result of SAGE GENIE by visualizing the strength of blot and mediator complex 20 was obtained as the mutant m-RNA product. GO and SEQUENCE TAG VIEWER TOOL were used to get the relevant information of the MED-20 tag and to retrieve the nucleotide sequence of the MED-20.Finally pairwise alignment was done in between normal nucleotide sequence of chromosome 6 and mutant MED-20 tag by EMBOSS TOOL and was identified, as a single nucleotide mutation at guanine. Further Comparative protein modeling can be done for MED-20 and its docking studies can be done to identify better drugs which can stop the over expression of the gene

Topics: CGAP, Lung Cancer, Small lung Carcinoma, Emboss, SAGE, EST Tags, Northern Blot, Cancer
Year: 2010
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