Abstract A single biological control isolate belonging to the species aggregate Trichoderma harzianum Rifai was differentiated from other closely related Trichoderma species and commonly isolated mycoflora by molecular assay. Restriction fragment length polymorphism (RFLP) analysis enabled the differentiation of the single isolate; a hybridising band of 1.1 kb being diagnostic. Crosshybridisation occurred against the DNA from 12 Trichoderma isolates, representative of three species aggregates. The hybridisation probe used during the RFLP analysis was an amplification product produced during random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) analysis using DNA from T
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