Abstract Accurate measurement of vessel diameters on retinal images plays an important part in diagnosing cardiovascular diseases. In this study, a method of vessel diameter measurement has been developed incorporating with a tracking technique. Twin Gaussian functions are introduced to model the distribution of grey level profile over a vessel cross section. The tracking technique is utilised to study the variations of vessel diameter in the direction of vessel longitude axis. This enables us to obtain an average diameter over any length of a vessel and to develop more parameters for study of vascular diseases. 1
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