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Infection

By Lemaire Julien, Mkannez Ghada, Guerfali Fatma Z., Gustin Cindy, Attia Hanène, Sghaier Rabiaa M., Dellagi Koussay, Laouini Dhafer and Renard Patricia

Abstract

have developed sophisticated strategies to subvert the host macrophage responses. Despite a wealth of gained crucial information, these strategies still remain poorly understood. MicroRNAs (miRNAs), an evolutionarily conserved class of endogenous 22-nucleotide non-coding RNAs, are described to participate in the regulation of almost every cellular process investigated so far. They regulate the expression of target genes both at the levels of mRNA stability and translation; changes in their expression have a profound effect on their target transcripts.-infected human primary macrophages of three healthy donors assessed at different time-points post-infection (three to 24 h). We show that expression of 64 out of 365 analyzed miRNAs was consistently deregulated upon infection with the same trends in all donors. Among these, several are known to be induced by TLR-dependent responses. GO enrichment analysis of experimentally validated miRNA-targeted genes revealed that several pathways and molecular functions were disturbed upon parasite infection. Finally, following parasite infection, miR-210 abundance was enhanced in HIF-1α-dependent manner, though it did not contribute to inhibiting anti-apoptotic pathways through pro-apoptotic caspase-3 regulation. infection. These results could contribute to better understanding of the dynamics of gene expression in host cells during leishmaniasis

Publisher: Public Library of Science
Year: 2013
DOI identifier: 10.1371/journal.pntd.0002478
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