Skip to main content
Article thumbnail
Location of Repository

The Distribution of Caveolin-1 in Human Term Placenta and the Derivation of the Endothelial Cells Lining its Basal Plate

By Simon Byrne


The initial aim of this research was to establish the distribution of the protein caveolin-1\ud in the human term placenta, using an indirect immunofluorescence technique. The\ud findings of this survey showed that caveolin-1 was expressed in all the predicted places\ud (vascular endothelium, smooth muscle and fibroblasts), but also by cells lining the basal\ud plate. These cells were investigated further, firstly by transmission and scanning\ud electron microscopy, then by immuno-gold electron microscopy. The findings of the\ud immuno-gold study suggested a vectorial nature of caveolae trafficking in both foetal\ud endothelial cells and in the endothelial-like cells lining the basal plate. This study also\ud showed that some leucocytes express caveolin-1.\ud The cells lining the maternal blood space above the basal plate of the placenta had been\ud thought to be trophoblastic (foetal) but the initial results obtained using\ud immunofluorescence indicated that the lining consisted rather of a mosaic of two types\ud of cells. Some of these were trophoblastic but others were seen to be more similar to\ud endothelial cells.\ud To determine whether these latter cells were of maternal derivation, placentae from\ud neonates of known gender were used in a cytogenetic analysis, to establish their\ud provenance unequivocally. The probe used in these in situ hybridisation experiments\ud was complementary to a portion of the human Y-chromosome (only found in males).\ud Cells to which this probe hybridised must therefore be male; a failure to hybridise\ud meant that they must be maternal. Some cells resident in the basal plate were shown to\ud have more than one Y-chromosome in a single interphase nucleus and were taken to be\ud polyploid trophoblast ‘giant’ cells.\ud Further collaborative experiments showed that the area fraction occupied by the lining\ud endothelial cells was greater in pre-eclamptic placentae than in placentae from\ud normotensive births; the significance of this in the aetiopathology of this disease is\ud discussed.\ud Cells that play a key role at the maternal-foetal interface have been carefully\ud characterised in this study and have been shown to form a continuous\ud endothelial/epithelial layer of both maternal and foetal origin

Publisher: University of Leicester
Year: 2011
OAI identifier:

Suggested articles


  1. (2004). A confocal laser scanning microscope study of cytokeratin immunofluorescence dimming indicates a mechanism of trophoblast deportation and its upregulation in pre-eclampsia. doi
  2. (2003). A new method to enhance contrast of ultrathin cryosections for immunoelectron microscopy. doi
  3. (1990). A quantitative interference light microscope study of human first trimester chorionic villi. doi
  4. (2000). Adriana and Luisa Castellucci award lecture 1999: role of oxygen in the regulation of trophoblast gene expression and invasion. doi
  5. (1999). Angiogenesis activators and inhibitors differentially regulate caveolin-1 expression and caveolae formation in vascular endothelial cells. Angiogenesis inhibitors block vascular endothelial growth factor-induced down-regulation of caveolin-1. doi
  6. (2001). Angiogenesis and blood flow: implications for pathobiology: a workshop report. doi
  7. (1995). Behaviour of two IgG subclasses in transport of immunoglobulin across the human placenta.
  8. (2003). Caveolae and caveolin-1 in human term villous trophoblast. doi
  9. (1999). Caveolin-1 expression and caveolae biogenesis during cell transdifferentiation in lung alveolar epithelial primary cultures. doi
  10. (1997). Cell-type and tissue-specific expression of caveolin-2. doi
  11. (2000). Chronic intrauterine bleeding and fetal growth at less than 32 weeks of gestation. doi
  12. (1993). Confocal and conventional immunofluorescence and ultrastructural localisation of intracellular strength giving components of human fetal membranes. doi
  13. (1998). Confocal microscopy: theory and applications. doi
  14. (1997). Dissecting the interaction between nitric oxide synthase (NOS) and caveolin. doi
  15. (1995). Editorial: The confocal laser scanning microscope (CLSM). doi
  16. (1995). Endothelial dysfunction yes, cytotoxicity no! doi
  17. (1998). Etiology and pathogenesis of preeclampsia. workshop report. Trophoblast Res 16:S142–S145.
  18. (1995). Expression of Cdx-2 in the mouse embryo and placenta: possible role in patterning of the extra-embryonic membranes. Dev Dyn 204:219–227. doi
  19. (1999). Extracellular simian virus-40 transmits a signal that promotes virus enclosure within caveolae. doi
  20. (2003). Failure of physiologic transformation of the spiral arteries in patients with preterm labor and intact membranes. doi
  21. (2003). Failure of physiologic transformation of the spiral arteries in the placental bed in preterm premature rupture of membranes. doi
  22. (1993). Fine Structure Immunocytochemistry. doi
  23. followed by mounting under a No. 0 coverslip in a photobleach retardant, glycerol-based mountant (Citifluor,
  24. (1998). Gestational age-dependent extravillous cytotrophoblast osteopontin immunolocalization differentiates between normal and preeclamptic pregnancies. doi
  25. (2004). Healthy and pre-eclamptic placental basal plate lining cells: quantitative comparisons based on confocal laser scanning microscopy. doi
  26. (1992). Hypertension in pregnancy. doi
  27. (2001). Hypoxia implications for implantation to delivery—a workshop report. doi
  28. (2001). Immunocytochemical localisation of a caveolin-1 isoform in human term extra-embryonic membranes using confocal laser scanning microscopy: implications for the complexity of the materno-fetal junction. doi
  29. (1998). Immunocytochemical localisation of Caveolin-1 in human term extraembryonic membranes using confocal laser scanning microscopy. doi
  30. (2001). Immunocytochemical Localisation of Caveolin-1 in human term extraembryonic membranes: implications for the complexity of the materno-fetal junction. doi
  31. (2001). Immunocytochemical localization of endothelin-1 in human placenta from normal and pre- eclamptic pregnancies. Hypertens Preg 20:125–137. doi
  32. (1993). Immunohistochemical evidence for the heterogeneity of maternal and fetal vascular endothelial cells in human full-term placenta. Cell Tissue Res 274:211–218. doi
  33. (2001). Key regulatory factors involved in placental development: a review. Placenta 22:S83– S92. doi
  34. (1990). Localisation of cytoskeletal proteins in chorionic villi.
  35. (1999). Maternal central hemodynamics in hypertensive disorders of pregnancy. doi
  36. (1981). Morphogenesis of human placental chorionic villi: cytoskeletal, syncytioskeletal and extracellular matrix proteins. doi
  37. (2003). Myometrial and placental artery reactivity alone cannot explain reduced placental perfusion in preeclampsia and intrauterine growth restriction. doi
  38. (2002). Natural killer cells and pregnancy. doi
  39. (2002). Nostrin: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. doi
  40. (2001). Nutrition of the human fetus during the first trimester: a review. Placenta 22:S70– S76. doi
  41. (2000). Pathology of the human placenta. Early development of the human placenta, 4th ed. doi
  42. (2001). Phenotype of the endothelium in the human term placenta. doi
  43. (2000). Placental transporters relevant to drug distribution across the maternal–fetal interface.
  44. (2001). Plasminogen activators and inhibitors are transcribed during early macaque implantation. doi
  45. (2004). Pre-Eclamptic Placental Basal Plate Lining Cells: Quantitative Comparisons Based on Confocal Laser Scanning Microscopy R.K. doi
  46. (1997). Scanning Microscopy Sections were studied using a confocal laser scanning attachment (Bio-RadMRC 600) linked to a Zeiss Axiovert Balducci
  47. (2001). Severely reduced presence of tissue macrophages in the basal plate of pre-eclamptic placentae. doi
  48. (2001). Signal transductions: variants on developmental control from implantation to delivery. Placenta 22:S98–S100. doi
  49. (1997). Sterology and its impact on our understanding of human placental functional morphology. doi
  50. (1984). Syncytioskeletons in choriocarcinoma in culture.
  51. (1997). Techniques of advanced light microscopy and their applications to morphological analysis of human extra-embryonic membranes. doi
  52. (1998). The caveolae membrane system. doi
  53. (1988). The classification and definition of the hypertensive disorders of pregnancy. doi
  54. (1955). The fine structure of the gall bladder epithelium of the mouse. doi
  55. (1970). The human placenta. doi
  56. (2002). The isoform of caveolin-1 is a marker of vasculogenesis in early lung development. doi
  57. (2002). The regional expression of insulin-like growth factor II (IGF-II) and insulin-like growth factor binding protein-1 (IGFBP-1) in the placentae of women with preeclampsia. doi
  58. (2002). The role of the placenta in pre-eclampsia. doi
  59. (2006). Ultrahigh-resolution immunoRothberg,
  60. (2003). Ultrathin cryosections: an important tool for immunofluorescence and correlative microscopy. doi
  61. (1999). VEGF induces nuclear translocation of Flk-1/KDR, endothelial nitric oxide synthase and caveolin-1 in vascular endothelial cells. doi
  62. (1966). Wird bei der Geburtsplacenta des Menschen die Basalplatte von Trophoblastzellen oder Zellen mu¨tterlicher herkunft u¨berzogen. Acta Anat 63:545–558. 62 R.K. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.