Skip to main content
Article thumbnail
Location of Repository

Applications of accelerator mass spectrometry for pharmacological and toxicological research

By Karen Brown, Elaine M. Tompkins and Ian N.H. White

Abstract

This paper was published as Mass Spectrometry Reviews, 2006, 25 (1), pp. 127-145. It is available from http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1098-2787. DOI: 10.1002/mas.20059Metadata only entryThe technique of accelerator mass spectrometry (AMS), known for radiocarbon dating of archeological specimens, has revolutionized high-sensitivity isotope detection in pharmacology and toxicology by allowing the direct determination of the amount of isotope in a sample rather than measuring its decay. It can quantify many isotopes, including 26Al, 14C, 41Ca, and 3H with detection down to attomole (10−18) amounts. Pharmacokinetic data in humans have been achieved with ultra-low levels of radiolabel. One of the most exciting biomedical applications of AMS with 14C-labeled potential carcinogens is the detection of modified proteins or DNA in tissues. The relationship between low-level exposure and covalent binding of genotoxic chemicals has been compared in rodents and humans. Such compounds include heterocyclic amines, benzene, and tamoxifen. Other applications range from measuring the absorption of 26Al to monitoring 41Ca turnover in bone. In epoxy-embedded tissue sections, high-resolution imaging of 14C label in cells is possible. The uses of AMS are becoming more widespread with the availability of instrumentation dedicated to the analysis of biomedical samples

Topics: accelerator mass spectrometry, radiolabel, microdosing, pharmacokinetics, DNA binding
Publisher: John Wiley & Sons
Year: 2005
DOI identifier: 10.1002/(ISSN)1098-2787
OAI identifier: oai:lra.le.ac.uk:2381/9499
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://hdl.handle.net/2381/949... (external link)
  • http://onlinelibrary.wiley.com... (external link)
  • http://dx.doi.org/10.1002/mas.... (external link)
  • http://onlinelibrary.wiley.com... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.