Skip to main content
Article thumbnail
Location of Repository

The molecular characterisation of narcissus latent virus and Maclura mosaic virus

By Joanne Louise Badge


The published articles from Appendix III (p. 133 to end) are not available in the electronic version of this thesis due to copyright restrictions. The full version can be consulted at the University of Leicester Library.Narcissus latent virus (NLV) and Maclura mosaic virus (MacMV) are serologically related. However, they have poor serological relationships with other plant viruses with which they have shared characteristics. Coat protein size, particle shape and structure, mode of vector transmission, cytology and serology proved insufficient to classify them. Molecular techniques were employed in order to create tools for the rapid and accurate classification of plant viruses. A carlavirus-specific PCR primer test failed to amplify NLV or MacMV but confirmed that several other viruses belonged to the carlavirus genus.\ud The nucleotide sequences of part of the nuclear inclusion body (NIb) gene, the complete coat protein gene and the 3' untranslated regions of narcissus latent virus (NLV) and Maclura mosaic virus (MacMV) were determined. Deduced amino acid sequences for the NIb and coat protein genes revealed that NLV and MacMV are closely related.\ud Comparison of the NIb sequences with other viruses showed that NLV and MacMV have closer affinities with viruses of the Potyviridae than to those of the carlavirus genus with which they were initially classified. It is proposed that NLV and MacMV may form a new genus within the Potyviridae, the Macluraviruses. The viruses associated with narcissus yellow stripe disease were re-evaluated.\ud In order to identify further members for the new genus a second PCR primer was designed to amplify a region shared by the bymoviruses and macluraviruses. Sequence data obtained for the 3'-terminal region of rice necrosis mosaic virus (RNMV) using a fragment amplified by this primer confirmed that RNMV was a member of the bymovirus genus. MacMV and barley mild mosaic bymovirus replicase sequences were used to transform tobacco plants in an attempt to create transgenic resistance

Publisher: University of Leicester
Year: 1997
OAI identifier:

Suggested articles


  1. (1997). A bymovirus-specific PCR primer and partial nucleotide sequence provides further evidence for the recognition of rice necrosis mosaic virus as a bymovirus.
  2. (1996). A carlavirusspecific PCR primer and partial nucleotide sequence provides further evidence for the recognition of cowpea mild mottle virus as a whitefly-transmitted carlavirus.
  3. (1990). A comparison of the nucleotide sequence homologies between isolates of the Andean and ordinary strains of potato virus S and their relationship to other carlaviruses. Virus Genes,
  4. (1992). A defective replicase gene induces resistance to cucumber mosaic virus in transgenic tobacco plants.
  5. (1990). A point mutation in the coat protein abolishes aphid transmission of a potyvirus.
  6. (1993). A polymerase chain reaction method adapted for selective amplification and cloning of 3' terminal sequences of potyviral genomes : application to mosaic virus.
  7. (1980). A review of problems and progress in research on viruses and virus diseases of narcissus in Britain.
  8. (1989). A second proteinase encoded by a plant potyvirus genome.
  9. (1992). A summary of potyvirus taxonomy and definitions.
  10. (1962). A system of viruses.
  11. (1988). a). An appraisal of the identities of potyviruses infecting narcissus.
  12. (1988). Amino acid sequence homology of coat protein as a basis for identification and classification of the potyvirus group.
  13. (1991). Analysis of the coat proteins of the ordinary and Andean strains of potato virus S and antigenic comparisons of carlaviruses.
  14. (1992). Approaches to nonconventional control of plant virus disease.
  15. (1995). Autocatalytic processing of the 223-kDa protein of blueberry scorch carlavirus by a papain-like proteinase.
  16. (1988). b). Narcissus late season yellows potyvirus: symptoms, properties and serological detection.
  17. (1990). Barley yellow mosaic virus (BaYMV) and BaYMV-M: two different viruses.
  18. (1990). Barley yellow mosaic virus and barley mild mosaic virus: strains and host resistance.
  19. (1975). Barley yellow mosaic virus.
  20. (1993). Carlavirus isolates from cultivated Allium species represent three viruses.
  21. (1996). Cassava brown streak associated (? ) carlavirus.
  22. (1986). Cassava viruses from Africa. Report of the Scottish Crop Research Institute
  23. (1991). cDNA cloning and nucleotide sequence of wheat streak capsid protein gene.
  24. (1991). Cell-free translation of American hop latent virus.
  25. (1993). Characterisation of potyviruses from tulip and lily which cause flower-breaking.
  26. (1992). Characterisation of the potyviral HC-Pro autocatalytic cleavage site.
  27. (1997). Classification and nomenclature of viruses. Fifth Report of the International Committee on Taxonomy of Viruses.
  28. (1970). Cleavage of the structural proteins during the assembly of the head of bacteriophage T4.
  29. (1995). Cloning and sequence analysis of RNA-2 of a mechanically transmitted UK isolate of barley mild mosaic bymovirus (BaMMV).
  30. (1993). Cloning and sequence analysis of the coat protein gene of barley mild mosaic virus.
  31. (1992). Coat protein phylogeny and systematics of potyviruses.
  32. (1990). Comparative sequence of the helper component (HC) region of potato virus Y and a HC-defective strain, potato virus C. Virology 178,573-578.
  33. (1991). Comparison and differentiation of potyvirus isolates and identification of strain-, virus-, and subgroup-specific and potyviruscommon epitopes using monoclonal antibodies.
  34. (1995). Complementation of tobacco etch potyvirus mutants by active RNA polymerase expression in transgenic cells.
  35. (1990). Conservation of the 3' terminal nucleotide sequence in five carlaviruses.
  36. (1973). Cowpea mild mottle, a newly recognised virus infecting cowpeas (Vigna unguiculata) in Ghana.
  37. (1994). Cytoplasmic inclusions induced by narcissus latent virus.
  38. (1997). Delayed resistance to plum pox potyvirus mediated by a mutated RNA replicase gene: involvement of a gene-silencing mechanism.
  39. (1989). Deployment of resistance to the barley yellow mosaic virus in the UK.
  40. (1990). Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence.
  41. (1966). Effects of infection with narcissus mosaic, narcissus yellow stripe and tobacco rattle viruses on the growth and flowering of narcissus cv. 'Minster Talma'.
  42. (1991). Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive strand RNA viruses.
  43. (1991). Evidence for subgenomic RNAs in leaves infected with an Andean strain of potato virus S.
  44. (1990). Evidence for the role of subgenomic RNAs in the production of potato virus S coat protein during in vitro translation.
  45. (1990). Evidence for the role of subgenoniic RNA species in the production of Helenium virus S coat protein during in vitro translation.
  46. (1993). Evolution and taxonomy of positive strand RNA viruses: implications of comparative analysis of amino acid sequences.
  47. (1988). Evolution of plus-strand RNA viruses.
  48. (1989). Evolutionary relationship between luteoviruses and other RNA plant viruses based on sequence motifs in their putative RNA polymerases and nucleic acid helicases.
  49. (1943). Experiments in the spread of "narcissus stripe" in the field.
  50. (1988). Expression and function of potyviral gene products. Annual Review of Phytopathology
  51. (1992). Expression of amino-acid terminal portions or full length viral replicase genes in transgenic plants confers resistance to potato virus X infection.
  52. (1986). Expression of biologically active viral satellite RNA from the nuclear genome of transformed plants.
  53. (1993). Extreme resistance to potato virus X infection in plants expressing a modified component of the putative viral replicase.
  54. (1987). Functional inactivation of a gene by dominant negative mutations.
  55. (1996). Fungal transmission of plant viruses.
  56. (1996). Genomic reassortment of barley mild mosaic virus : evidence for involvement of RNA 1 in pathogenicity.
  57. (1981). Handbook of plant virus infections comparative diagnosis (E. Ku stak).
  58. (1992). Highlights and prospects of potyvirus molecular biology.
  59. (1990). Homologies between the genomes of a carlavirus (lily symptomless virus) and a potexvirus (lily virus X) from lily plants.
  60. (1995). Homology-dependent resistance: transgenic virus resistance in plants related to homology-dependent gene silencing.
  61. (1993). Identification and classification of a resistance breaking strain of barley yellow mosaic virus.
  62. (1985). Identification and symptom expression of four elongated viruses infecting bulbous iris. Acta Horticulturae
  63. (1989). Identification of essential residues in potyvirus proteinase HC-Pro by site-directed mutagenesis.
  64. (1989). Identification of four conserved motifs among the RNA-dependent polymerase encoding elements.
  65. (1991). Identification of potyviruses using the polymerase chain reaction with degenerate primers.
  66. (1996). Identification of structural similarities between putative transmission proteins of Polymyxa and Spongospora transmitted bymoviruses and furoviruses.
  67. (1987). Interference with viral infection by defective RNA polymerase.
  68. (1990). Interference with viral infection by RNA replicase deleted at the carboxy-terminal region.
  69. (1983). Intracellular occurrence of cowpea mild mottle virus in two unrelated plant species.
  70. (1990). Investigation of the 5' terminal structures of genomic and subgenomic RNAs of potato virus S. Virus Genes,
  71. (1946). Investigations on virus diseases of narcissus. Royal Horticultural Society, The Daffodil and Tulip Yearbook.
  72. (1978). Light and electron microscopy of cells infected with maclura mosaic virus.
  73. (1964). Localisation of viral antigen in narcissus leaves infected with yellow stripe virus, determined by means of a fluorescein conjugated antiserum.
  74. (1979). Maclura mosaic virus - an elongated plant virus of uncertain classification.
  75. (1981). Madura mosaic virus.
  76. (1993). Molecular analysis of the capsid protein of a German isolate of barley mild mosaic virus.
  77. (1996). Molecular cloning and nucleotide sequencing of the 3'-terminal region of a South African isolate of ryegrass mosaic virus RNA and in vitro expression of the coat protein gene.
  78. (1996). Molecular cloning and nucleotide sequencing of the partial genomes of Agropyron and Hordeum mosaic viruses, two members of the Rymovirus genus in the taxonomic family Poryviridae.
  79. (1996). Molecular evidence that white-fly transmitted sweet potato mild mottle virus belongs to a distinct genus of the Potyviridae.
  80. (1989). Molecular genetic analysis of a plant virus polyprotein cleavage site: a model.
  81. (1995). Molecular systematics of the Potyviridae, the largest plant virus family.
  82. (1995). Movement of bymoviruses and functions of RNA2-encoded proteins of barley yellow mosaic virus.
  83. (1995). Mutational analysis of the coat protein N-terminal amino acids involved in potyvirus transmission by aphids.
  84. (1976). Narcissus latent virus.
  85. (1991). Narcissus latent, a virus with filamentous particles and a novel combination of properties.
  86. (1932). Narcissus mosaic symptoms.
  87. (1966). Narcissus mosaic virus.
  88. (1971). Narcissus yellow stripe virus.
  89. (1991). Nucleotide sequence and gene organisation of the 3'-terminal region of chrysanthemum virus B genomic RNA.
  90. Nucleotide sequence of barley yellow mosaic virus RNA 1: a close evolutionary relationship with potyviruses.
  91. (1991). Nucleotide sequence of barley yellow mosaic virus RNA 2.
  92. (1989). Nucleotide sequence of narcissus mosaic virus RNA.
  93. (1989). Nucleotide sequence of potato virus Y (N strain) genomic RNA.
  94. (1991). Nucleotide sequence of the 3' terminal region of lettuce mosaic potyvirus RNA shows a Gln/Val dipeptide at the cleavage site between the polymerase and the coat protein.
  95. (1991). Nucleotide sequence of the 3'-terminal region of carnation latent virus.
  96. (1990). Nucleotide sequence of the 3'-terminal region of Helenium virus S.
  97. (1989). Nucleotide sequence of the capsid protein gene of barley yellow mosaic virus.
  98. (1994). Nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases.
  99. (1975). Oat mosaic virus.
  100. (1991). Occurrence of chloroplast ribosome binding recognition sites within conserved elements of the RNA genomes of carlaviruses.
  101. (1989). Organisation and interviral homologies of the 3'-terminal portion of potato virus S.
  102. (1992). Partial nucleotide sequence of poplar mosaic virus RNA confirms its classification as a carlavirus.
  103. (1989). Partial nucleotide sequence of potato virus M RNA shows similarities to potexviruses in gene arrangement and the encoded amino acid sequences.
  104. (1995). Pathogen-derived resistance to plant viruses.
  105. (1977). Peptide mapping by limited proteolysis in sodium dodecyl suplhate and analysis by gel electrophoresis.
  106. (1996). Phylogenetic justification for splitting the Rymovirus genus of the taxonomic family Potyviridae.
  107. (1992). Plants transformed with a region of the 201-kilodalton replicase gene of pea early browning virus are resistant to infection.
  108. (1990). Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus.
  109. (1986). Potential metal binding domains in nucleic acid binding proteins.
  110. (1991). Potyviridae, a proposed family of plant viruses.
  111. (1987). Potyvirus proteins share amino acid sequence homology with Picorna-, Como- and Caulimoviral proteins.
  112. (1984). Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses.
  113. (1995). Primary structure and sequence analysis of RNA2 of a mechanically transmitted barley mild mosaic virus isolate: an evolutionary relationship between bymo- and furoviruses.
  114. (1989). Production of antiserum to a non-structural potyviral protein and its use to detect narcissus yellow stripe and other potyviruses.
  115. (1993). Progress towards a higher taxonomy of viruses.
  116. (1985). Purification and characterisation of potyvirus helper component.
  117. (1967). Rapid detection of narcissus yellow stripe and two other filamentous viruses in crude negatively stained narcissus sap.
  118. (1992). Replicase-mediated resistance to plant viruses. Scientific Progress
  119. (1994). Replicasemediated resistance to potato virus Y in transgenic tobacco plants. Molecular PlantMicrobe Interactions,
  120. (1990). Resistance to barley yellow mosaic virus and to barley mild mosaic virus in barley.
  121. (1992). Resistance to tobacco mosaic virus induced by the 54kDa gene sequence requires expression of the 54kDa protein.
  122. (1977). Rice necrosis mosaic virus.
  123. (1990). RNA helicase: a novel catalytic activity associated with protein encoded by a positive-strand RNA virus.
  124. (1994). RNA-RNA recombination and evolution in virus-infected plants.
  125. (1961). Selective cleavage of the methionyl peptide in ribonuclease cyanogen bromide.
  126. (1994). Sequence analysis of the 3' terminal half of RNA 1 of wheat spindle streak mosaic virus.
  127. (1992). Sequence analysis of the 3' terminal halves of RNA-1 of two strains of barley mild mosaic virus.
  128. (1995). Sequence of the 3'-part of the RNA of ryegrass mosaic virus, a potyvirus.
  129. (1995). Sequence of the 3'-terminal region of the RNA of a mite transmitted potyvirus from Hordeum murinum L.
  130. (1990). Sequence of the 3'-terminal region of turnip mosaic virus RNA and the capsid protein gene.
  131. (1992). Site-directed mutations in the potyvirus HC-Pro gene affect helper component activity, virus accumulation and symptom expression in infected tobacco plants.
  132. (1971). Sixteen groups of plant viruses.
  133. (1996). Some characteristics of a virus infecting Solanum tuberosum cv. Red La Soda imported from the USA. Scottish Agricultural Science Agency, East Craigs,
  134. (1977). Some hosts and properties of narcissus latent virus, a carlavirus commonly infecting narcissus and bulbous iris.
  135. (1989). Some properties of nucleic acids and coat proteins of soil-borne filamentous viruses.
  136. (1939). Studies on narcissus mosaic.
  137. (1992). The 3'-nucleotide sequence of an ordinary strain of potato virus S. Virus Genes,
  138. (1991). The 35-kDa protein from the N-terminus of the potyviral polyprotein functions as a third virus-encoded proteinase.
  139. (1982). The Carlavirus Group.
  140. (1990). The classification of organisms at the edge of life or Problems with virus systematics.
  141. (1996). The complete nucleotide sequence and genome organisation of barley mild mosaic virus (Nal strain).
  142. (1995). The complete nucleotide sequence and genome organisation of the mite-transmitted brome streak mosaic virus in comparison with those of potyviruses.
  143. (1996). The complete nucleotide sequence of barley mild mosaic virus RNA1 and its relationship with other members of the Potyviridae.
  144. (1989). The complete nucleotide sequence of plum pox virus RNA.
  145. (1992). The complete nucleotide sequence of RNA 1 of a German isolate of barley yellow mosaic virus and its comparison with a Japanese isolate.
  146. (1994). The complete nucleotide sequence of RNA 2 of barley mild mosaic virus (BaMMV).
  147. (1996). The complete nucleotide sequence of RNA-2 of a fungally-transmitted isolate of barley mild mosaic virus and identification of amino acid combinations possibly involved in fungus transmission.
  148. (1985). The concept of parasite-derived resistance: deriving resistance genes from the parasites own genome.
  149. (1992). The general properties of potyviruses. In:
  150. (1991). The genome organisation of potato virus M RNA.
  151. (1993). The natural occurrence, transmission, properties and possible affinities of cowpea mild mottle virus.
  152. (1991). The nucleotide sequence of RNA 2 of barley yellow mosaic virus.
  153. (1986). The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein.
  154. (1986). The nucleotide sequence of tobacco vein mottling virus RNA.
  155. (1994). The Potyviridae.
  156. (1992). The structure and expression of the genome of carlaviruses.
  157. (1982). The use of F(ab') 2-based ELISA to detect serological relationships among carlaviruses.
  158. (1991). Translation of potato virus S RNA in vitro evidence of protein processing.
  159. (1973). Two viruses isolated from Maclura pomifera.
  160. (1894). Variegation in narcissus.
  161. (1985). Viral Taxonomy for the nonvirologist.
  162. (1995). Virus and virus-like diseases of bulb and flower crops.
  163. (1990). Virus diseases.
  164. (1990). Virus species, a much overlooked but essential concept in virus classification.
  165. (1976). Wheat yellow moasic virus.
  166. (1987). Zinc-Fingers': a novel protein motif for nucleic acid recognition. Trends

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.