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Inhibition of the contractile response of the rat detrusor muscle by the β2-adrenoceptor agonist clenbuterol

By Diane Hudman, Ruth A. Elliott and Robert I. Norman

Abstract

This paper was published as European Journal of Pharmacology, 2000, 392 (1-2), pp. 79-85. It is available from http://www.sciencedirect.com/science/journal/00142999. Doi: 10.1016/S0014-2999(00)00107-2Metadata only entryThe action of clenbuterol, β2-adrenoceptor agonist, on the contractile response of isolated rat detrusor muscle strips was investigated in vitro. Clenbuterol (10−5 M) inhibited the detrusor muscle frequency response (1–40 Hz, p<0.02) with a more pronounced effect at 1 Hz than 40 Hz. Clenbuterol (10−6 M) significantly inhibited the contractile response to exogenous ATP (10−4 to 10−2 M, p<0.05) but not to carbachol (10−9 to 10−4 M). The presence of 10−5 M ICI 118, 551, β2-adrenoceptor antagonist, shifted significantly the clenbuterol dose–response to 1 Hz electrical field stimulation (EC50 3.4×10−6 M (±2.2×10−6 M) for clenbuterol alone, to 4.1×10−4 M (±8.8 ×10−5 M), P<0.05). In conclusion, clenbuterol inhibits electrical field and ATP-stimulated contractions of detrusor muscle. Reversal of the clenbuterol inhibition of detrusor muscle contraction by ICI 118, 551 shows that clenbuterol is probably acting through postsynaptic β2-adrenoceptors, which modulate the response to ATP released from purinergic nerves

Publisher: Elsevier
Year: 2000
DOI identifier: 10.1016/S0014-2999(00)00107-2
OAI identifier: oai:lra.le.ac.uk:2381/8946
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