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Minisatellite Variants Generated in Yeast Meiosis Involve DNA Removal During Gene Conversion

By Alexander J.R. Bishop, Edward J. Louis and Rhona H. Borts

Abstract

The full-text of this article is not available on the LRA. The original published version is available from the publisher's website at:\ud http://www.genetics.org/Two yeast minisatellite alleles were cloned and inserted into a genetically defined interval in Saccharomyces\ud cerevisiae. Analysis of flanking markers in combination with sequencing allowed the determination of\ud the meiotic events that produced minisatellites with altered lengths. Tetrad analysis revealed that gene\ud conversions, deletions, or complex combinations of both were involved in producing minisatellite variants.\ud Similar changes were obtained following selection for nearby gene conversions or crossovers among random\ud spores. The largest class of events involving the minisatellite was a 3:1 segregation of parental-size\ud alleles, a class that would have been missed in all previous studies of minisatellites. Comparison of the\ud sequences of the parental and novel alleles revealed that DNA must have been removed from the recipient\ud array while a newly synthesized copy of donor array sequences was inserted. The length of inserted\ud sequences did not appear to be constrained by the length of DNA that was removed. In cases where one\ud or both sides of the insertion could be determined, the insertion endpoints were consistent with the\ud suggestion that the event was mediated by alignment of homologous stretches of donor/recipient DNA

Publisher: Genetics Society of America
Year: 2000
OAI identifier: oai:lra.le.ac.uk:2381/8798
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