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Analysis of Meiotic Recombination Products from Human Sperm

By Liisa Kauppi, Celia A. May and Alec J. Jeffreys

Abstract

This is the author's final draft of the paper published as Methods in Molecular Biology, 2009, 557, pp. 323-355. The original publication is available at www.springerlink.com. Doi: 10.1007/978-1-59745-527-5_20Traditional methods for surveying meiotic recombination in humans are limited to pedigree and linkage disequilibrium analyses. We have developed assays that allow the direct detection of crossover and gene conversion molecules in batches of sperm DNA. To date, we have characterized 26 recombination hotspots by allele-specific PCR and selectively amplified recombinant DNA molecules from these regions. These analyses have revealed that meiotic crossover hotspots in humans are highly localized and flanked by DNA segments where recombination is suppressed. The centers of crossover hotspots are also active in noncrossover recombination, displaying short conversion tracts

Publisher: Humana Press
Year: 2009
DOI identifier: 10.1007/978-1-59745-527-5_20
OAI identifier: oai:lra.le.ac.uk:2381/8105
Journal:

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Citations

  1. 1 Chapter 20 Analysis of Meiotic Recombination Products From Human Sperm Liisa Kauppi*, doi
  2. 46 Table 1. Recipe for 11.1x PCR buffer. Component Concentration of Volume Final Concentration Poisson-corr. number of crossovers
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