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Microbial diversity of Chinese lakes

By Eulyn Pagaling


Additional information available on CD in the print copy held at the University of Leicester Library.The microbial ecology of hot springs in Rehai geothermal field in Yunnan Province, China and salt lakes in Inner Mongolia, China were investigated. 16S ribosomal RNA (rRNA) genes were amplified from these environments and were cloned and sequenced. This molecular analysis showed that the 72.1'C hot spring contained Aquificae-dominated white streamers, while the hot water discharge (60 - 65*C) contained a laminated mat. This analysis also showed that the salt lakes were dominated by Halorubrum (34% of clone sequences). \ud The salt lakes' bacterial community consisted of Gammaproteobacteria(2 0%), Finnicutes (20%) and Bacteroidetes (20%), though the bacterium Salinibacter ruber appeared to be the predominant bacterium in the saltern at Lake Ejinnor. Moreover, several Gammaproteobacteria (67% of bacterial isolates) and Firmicutes (23%) and a few Archaea (Halorubrum and Haloarcula sp.) were cultivated from the salt lakes; the majority of isolates retrieved were not detected by molecular analysis. Statistical analysis showed that temperature, Na and Mg ions, and pH were the factors that drive microbial community composition in the salt lakes, and that geographic distance is not a factor, hence the hypothesis that 'the milieu selects' can be applicable. Furthermore, the archaeal community consists of closely related lineages, whereas the bacterial community is highly diverse.\ud Two novel archaeal viruses that infect a haloarchaeal strain that is 98% identical to Halontbrian saccharovorum1 6S rRNA sequencew ere isolated from salt lake water of Lake Bagaejinnor. Both are lytic head/tailed viruses; virus BJ1 is assigned to the Siphoviridae, while virus BJ2 is assigned to the Myoviridae, both of the order Caudovirales. Virus BJI has a 42271 base pair (bp) double stranded (ds) DNA genome, with a G+C content of 64.8 mol%\ud that is terminally redundant and may be circularly permuted. Almost all Open Reading Frames (ORFs) are in the forward strand in the same direction consistent with a rolling circle mechanism of DNA replication. A fraction of the virus population contained extra DNA that is probably host derived, and may suggest viral integration. Virus BJ2 genome is linear dsDNA. It is incomplete, but 97602 bp (approximately 90% of the genome) has been sequenced which has a G+C content of 51 mol% in 42 contigs. No genomici dentity between the viruses has been observed. Virus BJ2 has a narrow host range; it is unable to infect the type strain of Halorubrum saccharovorum or closely related field isolates. Virus BJ2 is stable from 4*C to 50'C, but titres drop ten fold at 60'C. Maximal titres were observed at 2 to 3M NaCl and titres were stable at pH 6- 10, but reduced 10000 fold at pH 4

Publisher: University of Leicester
Year: 2007
OAI identifier: oai:lra.le.ac.uk:2381/7662

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  1. (1991). 16S ribosomal DNA amplification for phylogenetic study. "
  2. (2007). 5500 Phages examined on the electron microscope. "
  3. (2002). A detailed phenotypic characterisation of the type strains of Halonionas species. "
  4. (2003). A gene encoding a novel extremely thermostable 1,4-beta-xylanase isolated directly from an environmental DNA sample. "
  5. (2005). A hundred years of Ainaliella research: 1905-2005. "
  6. (2005). A microbial arsenic cycle in a salt-saturated, extreme environment. "
  7. (1999). A molecular view of archacal diversity in marine and terrestrial hot water environments. "
  8. (1998). A natural view of microbial biodiversity within hot spring cyanobacterial mat communities. "
  9. (2002). A new phylum of Archaea represented by a nanosized hyperthermophilic symbiont. "
  10. (2005). A phylogenetic analysis of haloarchaea found in a solar saltern. "
  11. (1980). A square bacterium. "
  12. (2005). A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems. "
  13. (2006). A Voglesonger, et A
  14. (1972). Afethanobacterium thennoautotrophicus sp. n.. an anaerobic, autotrophic, extreme thermophile. "
  15. (2001). Alcalilimnicola halodurans gen. nov., sp. nov., an alkaliphilic, moderately halophilic and extremely halotolerant bacterium, isolated from sediments of soda-depositing Lake Natron, East Africa Rift Valley. "
  16. (2004). Alkaliflexits imshenetskii gen. nov. sp. nov., a new alkaliphilic gliding carbohydrate-fermenting bacterium with propionate fon-nation from a soda lake. "
  17. (1994). Alkaliphiles: diversity and identification. " Bacteria] Diversity and Systematics.
  18. (1999). Alkaliphiles: some applications of their products for biotechnology. "
  19. (1980). An alkaliphilic red halophilic bacterium with a low magnesium requirement from a Kenyan soda lakc. "
  20. (1988). An Australian halobacterium contains a novel proton pump retinal protein: archaerhodopsin. "
  21. (1994). Anaerobic bacteria from hypersaline environments. "
  22. (1988). Anaerobic degradation of organic compounds at high salt concentrations. "
  23. (1994). Anaerobic degradation of toluene and o-xylene by a methanogenic consortium. "
  24. (2002). Anaerobic oxidation of arsenite in Mono Lake water and by a facultative, arsenite-oxidizing chemoautotroph, strain MLIJE-l. "
  25. (2004). Anaerobic, alkaliphilic, saccharolytic bacterium Alkalibacter saccharofermentans gen. nov., sp. nov. from a soda lake in the Transbaikal region of Russia. "
  26. (1974). Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. "
  27. (2005). Analysis of the virus population present in equine faeces indicates the presence of hundreds of uncharacterised virus genomes. "
  28. (2003). Analytic Rarefaction
  29. (1998). Analyzing genomes with cumulative skew diagrams. "
  30. (2001). Application of denaturing gradient gel electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular methods. "
  31. (1992). Archaea in coastal marine environments. "
  32. (2005). Archaeal and bacterial communities in geochernically diverse hot springs of Yellowstone National Park,
  33. (2005). Archaeal diversity along a soil salinity gradient prone to disturbance. "
  34. (2005). Archaeal diversity in tidal flat sediment as revealed by 16S rDNA analysis. "
  35. (2001). Archaeal diversity in waters from deep South African gold mines. "
  36. (2005). Archaeal genetics - the third way. "
  37. (1999). Arsenic in the Meager Creek hot springs environment,
  38. (2005). At least I in 20 16S rRNA sequence records currently held in public repositories is estimated to contain substantial anomalies. "
  39. (2001). Attempts to cultivate RLMO bacteria using site-specific water chemistry data,
  40. (2005). Bacillus bogoriensis sp. nov., a novel alkaliphilic halotolerant bacterium isolated from a Kenyan soda lake. "
  41. (1983). Bactcriophages of Halobacteriunt halobillm: virion DNAs and proteins. "
  42. (2001). Bacteria] community analysis of Indonesian hot springs. "
  43. (1999). Bacteria] diversity within the human subgingival crevice. "
  44. (1987). Bacteria] evolution. "
  45. (2005). Bacterial community shift along a subsurface geothermal water stream in a Japanese gold mine. "
  46. (2003). Bacterial diversity and sulfur cycling in a mesophilic sulfide-rich spring. "
  47. (2003). Bacterial diversity patterns along a gradient of primary productivity. "
  48. (1974). Bacteriophage of Halobacterium salinarium. "
  49. (1982). Bacteriophages of Halobacterium halobiunt: isolation from fermented fish sauce and primary characterisation. "
  50. (2006). Bacterioplankton community composition along a salinity gradient of sixteen high-mountain lakes located on the Tibetan plateau, China. "
  51. (1990). Basic local alignment tool. "
  52. (2005). Biodiversity and activity of the microbial community in the Kotelnikovsky Hot Springs (Lake Baikal). "
  53. (1999). Biofilm exopolymers control microbialite formation at thermal springs discharging into the alkaline Pyramid Lake,
  54. (2006). Biogeographical distribution and diversity of microbes in methane hydrate-bearing deep marine sediments on the Pacific Ocean Margin. "
  55. (2006). Biogeography of Prokaryotes in Ilypersaline Environments. Department of Genetics,
  56. (1993). Biology of DNA restriction"
  57. (1901). Bulletin del la Soci6t6 Vaudoisedes Sciences Naturelles.
  58. Calder6n-Paz, et aL (2000). "The microbial food web along salinity gradients. "
  59. Camakaris, et aL (2004a). "Combined use of cultivation-dependent and cultivation-independent methods indicates that members of most haloarchaeal groups in an Australian crystallizer pond are cultivable. "
  60. Camakaris, et aL (2004b). "Cultivation of Walsby's square haloarchaeon. "
  61. (2000). Castenholz
  62. (2003). Cellulose as extracellular polysaccharide of hot spring sulfur-turf bacterial mat. "
  63. (1990). Changes in lake levels, salinity and the biological community of Great
  64. (1997). Charactcrisation of Natronobacterillin niagadii phage (pChl, a unique archaeal phage containing DNA and RNA. "
  65. (1993). Characterisation of the dominant halophilic archaea in a bacterial bloom in the Dead Sea. "
  66. (2006). Characterisation ofTwo Novel I laloarchaeal Virusc. s
  67. (1996). Characterization of haloalkaliphilic strictly aerobic bacterium, isolated from Pantelleria island. "
  68. (1996). Chemical and Physical Context for Life in Terrestrial Hydrothermal Systems: Chemical Reactors for the Early Development of Life and Hydrothermal Ecosystems. "
  69. (1991). Chinese and Mongolian saline lakes: a limnological overview. "
  70. (1993). Chinese saline lakes. "
  71. (2001). Chloroflexi"'. In Bergey's Manual of Systematic Bacteriology.
  72. (2001). Class 1. Thermoprotei class. nov. "
  73. (2001). Class 111. Halobacteria class nov. "
  74. (2001). Class IV. Thermococci class. nov. "
  75. (2001). Class IV. Thermoplasmata class. nov. "
  76. (2001). Class VIL "Methanopyri
  77. (2003). Climatology and trends of the temporal and spatial distribution of sandstorms in Inner Mongolia. " Water. Air and Soil Pollution: Focus
  78. (2005). Clostridium alkalicellum sp. nov., an obligately alkaliphilic cellulolYtic bacterium from a soda lake in the Baikal Region. "
  79. (2005). Community composition of a hypersaline endoevaporitic microbial mat. "
  80. (2002). Comparative 16S rRNA gene sequence analysis of microbial communities on recent Hawaiian volcanic deposits (1959,1921, 1790 & 1700): colonization and succession of microbes in extreme environments -Data
  81. (2001). Comparative metabolic diversity in two solar salterns. "
  82. (2001). Comparative phage genomics and the evolution of Siphoviridae: insights from dairy phages. "
  83. (2000). Comparisons of the polar profiles of two solar saltems located in
  84. (2000). Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacilhis subtilis. "
  85. (2006). Composition and dynamics of bacteria] communities of a drinking water supply system as assessed by RNA- and DNA-based 16S rRNA gene fingerprinting. "
  86. (2005). Constituents of SHI, a novel lipid-containing virus infecting the halophilic Euryarchaeon Haloarcula hispanica"
  87. (1991). Construction and use of halobactcrial shuttle vectors and further studies on Haloferar DNA gyrase. "
  88. (2001). Counting the uncountable: statistical approaches to estimating microbial diversity. "
  89. (1996). Cultivation of aerobic chemoorganotrophic Proteobacteria and Gram-positive bacteria from a hot spring microbial mat. "
  90. (1994). Definition and measurement of salinity in salt lakes. "
  91. (2000). Depositional facies and aqueous-solid geochemistry of travertine-depositing hot springs (Angel Terrace, Mammoth Hot Springs,
  92. (2003). Depth distribution of microbial diversity in Mono Lake, a meromictic soda lake in California. "
  93. (1983). Description of Deleya gen. nov. created to accommodate the marine species
  94. (1999). Desit1focella halophila gen. nov., sp. nov., a halophilic, fatty-acid-oxidizing, sulfate reducing bacterium isolated from sediments of the Great Salt Lake. "
  95. (1997). Dest(Ifonatronovibrio hydrogenovorans gen. nov., sp. nov., an alkaliphilic, sulfate-reducing bacterium. "
  96. (2003). Detecting cellulase and esterase enzyme activity encoded by novel genes present in environmental DNA. "
  97. (2005). Diel variations in carbon metabolism by green nonsulfur-like bacteria in alkaline siliceous hot spring microbial mats from Yellowstone National Park. "
  98. (2003). Differential growth response of colony-forming a- and yProteobacteria in dilution culture and nutrient addition experiments from Lake Kinneret (Israel), the Eastern Mediterranean Sea, and the Gulf of Eilat. "
  99. (1999). Direct analysis of genes encoding 16S rRNA from Rcfercnccs complex communities reveals many novel molecular species within the human gut. "
  100. (2004). Dissimilatory arsenate reduction with sulfide as electron donor: experiments with mono lake water and Isolation of strain MLMS-l, a chemoautotrophic arsenate respirer. "
  101. (2004). Distribution of prokaryotic genetic diversity in athalassohaline lakes of the Atacama Desert, Northern Chile. "
  102. (2004). Diversity and abundance of bacteria and archaea in the Bor Khlueng Hot Spring in Thailand. "
  103. (2003). Diversity and abundance of Crellarchaeola in terrestrial habitats studied by 16S rRNA surveys and real time PCR. "
  104. (2006). Diversity and ubiquity of thermophilic methanogenic archaea in temperate anoxic soils. "
  105. (2002). Diversity of Archaea in hypersaline environments characterized by molecular-phylogenetic and cultivation studies. "
  106. (2005). Diversity of halophilic archaca in the crystallizers of an Adriatic solar saltem. "
  107. (2002). Diversity of halophilic microorganisms: environments, phylogeny, physiology and applications. "
  108. (2004). Diversity of Kenyan soda lake alkaliphiles assessed by molecular methods. "
  109. (2007). Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland). "
  110. (2002). Diversity, abundance, and activity of archaeal populations in oil-contaminated groundwater accumulated at the bottom of an underground crude oil storage. "
  111. (1996). DNA recovery of soils of diverse composition. "
  112. (2004). Ecology and microbial structures of archaeal/bacterial strings-of-pearls communities and archaeal relatives thriving in cold suffidic springs. "
  113. (1992). Ecology, physiology and taxonomy studies on a new taxon of Haloanaerobiaceae, Haloincola saccharolytica gen. nov.,
  114. (1989). Ectothiorhodospira marismortid sp. nov., an obligately anaerobic, moderately halophilic purple sulfur bacterium from a hypcrsalinc sulfur spring on the shore of the Dead Sea. "
  115. el aL (2006b). "Halovivar asialicus gen. nov., sp. nov., a novel extremely halophilic archaeon isolated from Inner Mongolia, China. "
  116. (1986). Electrophoretic separations of large DNA molecules by periodic inversions of the electric field. "
  117. (1996). Endemism and biodiversity of thermophilic cyanobacteria.
  118. (2005). EsthnafeS: Statistical estimation of species richness and shared species from samples.
  119. (2006). et A
  120. et aL (1996a). "Natroniella acetigena gen. nov. sp. nov., an extremely haloalkaliphilic, homoacetic bacterium: a new member of Haloanaerobiales. "
  121. et aL (1996b). "Spirochaeta alk-alica sp. nov., Spirochaeta africana sp. nov., and Spirochaeta asiatica sp. nov., alkaliphilic anaerobcs from the continental soda lakes in central Asia and the East African Rift. "
  122. et aL (1997a). "Occurrence of virus-like particles in the Dead Sea. "
  123. et aL (1998a). "Moderately halophilic Gram-positive bacterial diversity in hypersaline environments. "
  124. et aL (1998b). "Biology of moderately halophilic aerobic bacteria. "
  125. et aL (1998b). "Microbial diversity of soda lakes. "
  126. et aL (2000b). "Diversity and community structure within anoxic sediment from marine salinity meromictic lakes and a coastal meromictic marine basin, Vestfold Hilds, Eastern Antarctica. "
  127. et aL (2002a). "Marinospirillum alk-aliphihinz sp. nov., a new alkaliphilic helical bacterium from Haoji soda lake in Inner Mongolia Autonomous Region of China. "
  128. et aL (2002b). "Salinicoccus alkaliphilits sp. nov., a novel alkaliphile and moderate halophile from Baer Soda Lake in Inner Mongolia Autonomous Region, China. "
  129. et aL (2003a). "Tindallia califomiensis sp. nov., a new anaerobic, haloalkaliphilic, spore-forming acetogen isolated from Mono Lake in California. "
  130. et aL (2003b). "Desuffonalronum thiodisnuttans sp. nov., a novel alkaliphilic, sulfate-reducing bacterium capable of lithoautotrophic growth "
  131. et aL (2004a). "Alkalimonas amylolytica gen. nov., sp. nov., and Alkalinionas delamerensis gen. nov., sp. nov., novel alkaliphilic bacteria from soda lakes in China and East Africa. "
  132. (2003). et aL (2004b). "Bacterial diversity of the Inner Mongolian Baer Soda
  133. et aL (2005a). "Viral diversity in hot springs of Pozzuoli, Italy, and characterisation of a unique archaeal virus, Acidianits bottle-shaped virus, from a new family, the Ampullaviridae. "
  134. et aL (2005b). "Independent virus development outside a host. "
  135. et aL (2005b). "Pontibaciffits marinits sp. nov., a moderately halophilic bacterium from a solar saltem, and emended description of the genus Pontibacillits. "
  136. et aL (2006a). "Halostagnicola larsenii gen. nov., sp. nov., an extremely halophilic archaeon from a saline lake in Inner Mongolia, China. "
  137. et aL (2006a). "Viruses of the archaea: a unifying view. "
  138. et aL (2006b). "Structural and genomic properties of the hyperthcrmophilic archaeal virus ATV with an extracellular stage of the reproductive cycle. "
  139. et aL (unpublished-b). Analysis of a bacterial community in a nearshore marine-cultural sediment, Chinese Academy of Sciences,
  140. et aL (unpublished-b). Protease-producing bacteria isolated from Arctic seawater,
  141. et aL (unpublished). Diversity of halophilic archaea
  142. (2001). et aL (unpublished). Naxosll, an extremely halophilic archaeon isolated from the island of Naxos, Greece,
  143. et aL (unpublished). Taxonomical position of a thermophilic bacterium, Caldotogafontana gen. nov., sp. nov, Chinese Academy of Sciences,
  144. (1989). et aL Molecular Clonina: A LaboratoEy Manudi.
  145. et at (2000). "A ncw facultativcly autotrophic hydrogcn- and sulfur-oxidizing bacterium from an alkaline environment. " 'Extremol2hiles
  146. (2006). Evidence for existence of "Mesotogas, " members of the order Thermotogales adapted to low-temperature environments. "
  147. (1999). Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage. "
  148. (2003). Experimental studies on New Zealand hot spring sinters: rates of growth and textural development. "
  149. (2000). Extreme Environments. "
  150. (2000). Extremely halophilic Bacteria in crystallizer ponds from solar salterns. "
  151. (2006). Extremely halophilic denitrifying bacteria from hypersaline inland lakes, Halovibrio denitrificans sp. nov. and Halospina denitrificans gen. nov., sp. nov., and evidence that the genus name Halovibrio Fendrich
  152. (1996). Fervidobacteritint gondwanense sp. nov., a new thermophilic anaerobic bacterium isolated from nonvolcanically heated geothennal waters of the Great Artesian Basin of Australia. "
  153. (2003). Filamentous anoxygenic phototrophs in hot springs. "
  154. (2003). Fingerprinting microbial assemblages from the oxic/anoxic chemocline of the Black Sea. "
  155. (1999). Friedmanniella spitmicola sp. nov. and Friedmanniella capsidata sp. nov. from activated sludge foam: gram-positive cocci that grow in aggregates of repeating groups of cocci. "
  156. (2005). Genetic and functional properties of uncultivated thermophilic crenarchaeotes from a subsurface gold mine as revealed by analysis of genome fragments. "
  157. (1999). Genetic diversity of archaea in deep-sea hydrothermal vent environments. "
  158. (2000). Genome sequence of Halobacterium species NRCL"
  159. (2004). Genome sequence of the deep-sea garnma-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy. "
  160. (2002). Genomic analysis of uncultured marine viral communities. "
  161. (1994). Geochernical structure and position of the Waiotapu geothermal field, New Zealand. "
  162. (2004). Glycerol metabolism in the extremely halophilic bacterium Salinibacter ruber. "
  163. (2006). Gracilibaciffits orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China. "
  164. (2005). Halalkalicoccus tibetensis gen. nov., sp. nov., representing a novel genus of haloalkaliphilic archaea. "
  165. (1995). Haloanaerobitim alcaliphihim sp. nov., an anaerobic moderate halophile from the sediments of Great
  166. (1995). Haloanaerobium lactisroseus sp. nov., an extremely halophilic fermentative bacterium from the sediments of a hypersaline lake, "
  167. (2003). Haloarchaeal viruses: how diverse are they? "
  168. (1999). Haloarcida quadrata sp. nov., a square, motile archaeon isolated from a brine pool in Sinai (Egypt). "
  169. (2005). Halobaciffits dabariensis sp. nov. and Halobacilhis aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. "
  170. (2006). Halobacterium jilantaiense sp. nov., a halophilic archaeon isolated from a saline lake in Inner Mongolia, China. "
  171. (1988). Halobacterium lacusprofundi sp. nov., a halophilic bacterium isolate from Deep Lake, Antarctica. "
  172. (1976). Halobacterium saccharovonint sp. nov., a carbohydrate-metabolising, extremely halophilic bacterium. "
  173. (1984). Halobacteroides halobitts gen. nov., sp. nov., a moderately halophilic anaerobic bacterium from the bottom sediments of the Dead Sea. "
  174. (1998). Halogeonietricum borinquense gen.
  175. (2002). Halomicrobium mukohataei gen. nov., comb. nov., and emended description of Halomicrobium mukohataei. "
  176. (1999). Halomonas campisalis sp. nov., a denitrifying, moderately haloalkaliphilic bacterium. "
  177. (2000). Halomonas magadii sp. nov., a new member of the genus Halomonas, isolated from a soda lake of the East African Rift Valley. "
  178. (2004). Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. "
  179. (2004). Halomonas ventosae sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium. "
  180. (1995). Halophage 1IF2: genome organisation and replication strategy. "
  181. (2007). Haloquadratum walsbyigen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltem crystallizers in Australia and Spain. "
  182. (2006). Halortibrum lipolyticum sp. nov. and Halortibruni aidingense sp. nov., isolated from two salt lakes in Xin-Jiang, China. "
  183. (2004). Halortibrum xingiiangense sp. nov., a novel halophile isolated from saline lakes in China. "
  184. (2002). Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. "
  185. (2004). Halotolerant aerobic heterotrophic bacteria from the Great Salt Plains of Oklahoma. "
  186. (2004). Haloviruses 11171 and 11172: evidence for a recent and large recombination event. "
  187. (1993). HFI and IIF2: novel bacteriophages of halophilic archaea. "
  188. (2004). hi viro studies on putative shine-Dalgarno sequences Rcferenccs of the halophilic archaeon Halobacterium salinarium. "
  189. (2002). High bacterial diversity of a waste gas-degrading community in an industrial biofilter as shown by a 16S rDNA clone library. "
  190. (2000). High incidence of halotolerant bacteria in Pacific hydrothermal -vent and pelagic environments. "
  191. (2004). Highly efficient renaturation of beta-lactarnase isolated from moderately halophilic bacteria. "
  192. (2000). Highly ordered vertical structure of Synechococcits populations within the one-millimeter-thick photic zone of a hot spring cyanobacterial mat. "
  193. (2006). Hisl and His2 are distantly related, spindle-shaped haloviruses belonging to the novel virus group, Salterprovirus. "
  194. (1998). Hisl, an archaeal virus of the Fuselloviridde family that infects Haloarcula hispanica. "
  195. (2005). Hydrogen and bioenergetics in the Yellowstone geothermal ecosystem. "
  196. (2002). IIF2: a double-stranded DNA tailed haloarchaeal virus with a mosaic genome. "
  197. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the MI 3mp 18 and pUC 19 vectors. "
  198. (2003). Indigenous and contaminant microbes in ultradeep mines. "
  199. (2000). Influence of sulfide and temperature on species composition and community structure of hot spring microbial mats. "
  200. (1999). Inland saline lakes of Wadi El Natrun depression, Egypt "
  201. (1996). Insights into the volcanic arc mantle wedge from magnesian lavas from the Kamchatka arc. "
  202. (1994). International study on Artemia. New Survey of Arte"lia resources in the People's Republic of China. "
  203. (2005). Introducing DOTUR, a computer program for defining Operational Taxonomic Units and estimating species richness. "
  204. (2006). Introducing TreeClimber, a test to compare microbial community structures. "
  205. (2005). Investigation of the microbial ecology of intertidal hot springs by using diversity analysis of 16S rRNA and chitinase genes. "
  206. (1998). Isolation and characterisation of a novel facultatively alkaliphilic Nitrobacter species - Nb. alk-alicits sp. nov. "
  207. (1992). Isolation and Characterisation of Haloanaerobacter chitinovorans gen. nov., sp. nov., a halophilic, anaerobic, chitinolytic bacterium from a solar saltem. "
  208. (1991). Isolation and characterisation of Thiobaciffils halophihis sp. nov., a sulphur-oxidising autotrophic eubacteriurn from a Western Australian hypersaline lake. "
  209. (1991). Isolation and characterization of Desulfovibrio halophilits sp. nov., a halophilic sulfate-reducing bacterium isolated from Solar Lake (Sinai). "
  210. (1997). Isolation and characterization of halotolerant alkaliphilic methanotrophic bacteria from Tuva soda Lakes. "
  211. (2004). Isolation and cultivation of Walsby's square archaeon. "
  212. (2001). Isolation and properties of obligately chemolithotrophic and extremely alkalitolerant ammonia-oxidizing bacteria from Mongolian soda lakes. "
  213. (1979). Isolation and some properties of b-galactosidase producing bacteria. "
  214. (1988). Isolation of a halobacterial phage with a fully cytosine-methylated genome. "
  215. (2004). Isolation of haloarchaca that grow at low salinities. "
  216. Kuenen (2005a). "Chemolithotrophic haloalkaliphiles from soda lakes. "
  217. Kuenen (2005b). "Haloalkaliphilic sul fur-oxidi sing bacteria in soda lakes. "
  218. (2001). Lake status records from China: Data base document.
  219. (2005). Lentibacillits lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China. "
  220. (2004). Life at low water activity. "
  221. (1978). Life in high salt and solute concentrations. Microbial life in extreme environments.
  222. (1997). Light-induced motility of thermophilic Synechococcits isolates from Octopus Spring, Yellowstone National Park. "
  223. (2001). Magnetotel I uric images of deep crustal structure of the Rehai geothermal field near Tengchong, southern China. "
  224. (1991). Maintenance of Bacteria on Glass Beads at -60'C to -76C. "
  225. (2006). Marinimicrobium koreense gen. nov., sp. nov. and Marinimicrobium agarilyticum sp. nov., novel moderately halotolerant bacteria isolated from tidal flat sediment in Korea. "
  226. (1949). Measurement of diversity. "
  227. (1987). Mechanism of halotolerance in microorganisms. "
  228. (2004). MEGA 3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. "
  229. (2003). Metagenomic analyses of an uncultured viral community from human feces. "
  230. (1995). Methane consumption in (hyper)saline habitats of Crimea (Ukraine). "
  231. (2004). Methanogenic archaca and human periodontal disease. "
  232. (1988). Methanohalophilits zhilinae sp. nov., an alkaliphilic, halophilic, methylotrophic methanogen. "
  233. (2005). Methylohalobius crimeensis gen. nov., sp. nov., a moderately halophilic, methanotrophic bacterium isolated from hypersaline lakes of Crimea. "
  234. (1994). Microbial behaviour in salt-stressed ecosystems. "
  235. (1998). Microbial biofacies in hot-spring sinters; a model based on Ohaaki Pool, North Island, New Zealand. "
  236. (2004). Microbial biogeography along an estuarine salinity gradient: combined influences of bacteria] growth and residence time. "
  237. (2006). Microbial biogeography: putting microorganisms on the map. "
  238. (2006). Microbial biogeography? "
  239. (2003). Microbial communities associated with geological horizons in coastal subseafloor sediments from the Sea of Okhotsk. "
  240. (2006). Microbial community diversity associated with carbon and nitrogen cycling in permeable shelf sediments. "
  241. (2005). Microbial community genomics in the ocean. "
  242. (2005). Microbial community in a geothermal aquifer associated with the subsurface of the Great Artesian Basin, Australia. "
  243. (2004). Microbial diversity associated with a Paralvillella sulfincola tube and the adjacent substratum on an active deep-sea vent chimney. "
  244. (2000). Microbial diversity at 83*C in Calcite Springs, Yellowstone National Park: another environment where the Aquifcales and "Korarchaeota" coexist. "
  245. (2006). Microbial diversity in Maras saltems, a hypersaline environment in the Peruvian Andes. "
  246. (2005). Microbial diversity in production waters of a low-temperature biodegraded oil reservoir. "
  247. (2006). Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. "
  248. (2001). Microbial diversity of the brine-seawater interface of the Kebrit Deep, Red Sea, studied via 16S rRNA gene sequences and cultivation methods. "
  249. (2000). Microbial essentials at hydrothermal vents. "
  250. (1998). Microbial evolution, diversity and ecology: a decade of ribosomal RNA analysis of uncultivated microorganisms. "
  251. (2004). Microbiology at the Salt Plains Microbiology Observatory. Third Annual Microbial Observatories Principle Investigators Workshm.
  252. (1999). Molecular analysis of bacterial community structure and diversity in unimproved and improved upland grass pastures. " AM!
  253. (2005). Molecular analysis of the lower respirato[y tract microbiota. Infection, Immunity and Inflammation,
  254. (1999). Molecular characterisation of a toluene-degrading methanogenic consortium. "
  255. (2000). Molecular characterization of bacterial populations in petroleum-contaminated groundwater discharged from underground crude oil storage cavities. "
  256. (2002). Molecular characterization of novel red green nonsulfur bacteria from five distinct hot spring communities
  257. (2002). Molecular ecology of extremely halophilic Archaea and Bacteria. "
  258. (2003). Molecular sequence analysis of prokaryotic diversity in the anoxic sediments underlying cyanobacterial mats of two hypersaline ponds in Mediterranean salterns. "
  259. (2000). Monitoring complex bacterial communities using cultureindependent molecular techniques: application to soil environment. "
  260. (2001). Nalronobacterium nitratireducens sp. nov., a haloalkaliphilic archaeon isolated from a soda lake in China. "
  261. (2005). Nanoarchaea: representatives of a novel archaeal phylum or a fast-evolving euryarchaeal lineage related to Thermococcales? "
  262. (2001). Natrialba hithinbeirensis sp. nov. and Natrialba chahannaoensis sp. nov., novel haloalkaliphilic archaea from soda lakes in Inner Mongolia Autonomous region, China. "
  263. (2002). Natrialba magadii Virus qCh 1: first complete nucleotide sequence and functional organization of a virus infecting a haloalkaliphilic archaeon. "
  264. (1993). Natronobacterium vacitolata sp. nov., a haloalkaliphilic archaeon isolated from Lake Magadi, Kenya. "
  265. (1995). Natronococcits anzy1olyticits sp. nov., a haloalkaliphilic archaeon. "
  266. (1998). Natronoincola histidinovorans gen. nov., sp. nov., a new alkaliphilic acetogenic anaerobe. "
  267. (2005). Natronolimnobitts baerhitensis gen. nov., sp. nov. and Natronolininobitts innennongolicus sp. nov., novel haloalkaliphilic archaea isolated from soda lakes in Inner Mongolia,
  268. (2003). New approaches to analysing microbial biodiversity data. "
  269. (1990). Nitrogen fixation dynamics of two diazotrophic communities in
  270. (1984). Non-parametric estimation of the number of classes in a population. "
  271. nov., a novel halophilic archaeon from Puerto Rico. "
  272. (1999). Novel 16S rRNA gene sequences retrieved from highly saline brine sediments of kebrit deep, Red Sea. "
  273. (1999). Novel archaeal phylotypes from an East African alkaline saltern. "
  274. Novel bacterial and archaeal lineages from an in situ growth chamber deployed at a Mid-Atlantic Ridge hydrothermal vent. "
  275. (1998). Novel division level bacterial diversity in a Yellowstone hot spring. "
  276. (1998). Novel euryarchaeotal lineages detected on rice roots and in the anoxic bulk soil of flooded rice microcosms. "
  277. (2005). Obesity alters gut microbial ecology. "
  278. (1992). On the nature of global classification. "
  279. (1994). On the red coloration of saItcrn crystallizer ponds. 11. Additional evidence for the contribution of halobacterial pigments. "
  280. (2001). Order 1. Deinococcales. "
  281. (2001). Order 1. Methanobacteriales. "
  282. (2001). Order 11. Desulfurococcales. "
  283. (2005). Organic compatible solutes of halotolerant and halophilic microorganisms. "
  284. (1996). Perspectives on archaeal diversity, thermophily and monophyly from environmental rRNA Sequences. "
  285. (1994). Phylogenetic analysis of the hyperthermophilic pink filament community in Octopus Spring, Yellowstone National Park. "
  286. (2002). Phylogenetic approaches for describing and comparing the diversity of microbial communities. "
  287. (2006). Phylogenetic relationships within the order Halobacteriales inferred from 16S rRNA gene sequences. "
  288. (2002). Phylogeny of the family Halomonadaceae based on 23S and 165 rDNA sequence analyses. "
  289. (2001). Phylum 1311. Thermotogae phy. nov. "
  290. (2001). Phylum Al. Crenarchaeota
  291. (2001). Phylum All. Ettryarchaeota phy. nov. " In Berizey's Manual of Systematic Bacteriology.
  292. (2001). Phylum BIX. Deferribacteres phy. nov. "
  293. (2001). Phylum BVIL Thermornicrobia phy. nov. " In Bergey's Manual of Systematic Bacteriology.
  294. (2006). Planktonic microbial communities associated with fracturederived groundwater in a deep gold mine of South Africa. "
  295. (2006). Planktonic microbial community composition across steep physical/chemical gradients in pen-nanently ice-covered Lake Bonney, Antarctica. "
  296. (1982). Population dynamics of Dunaliella pana in the Dead Sea. "
  297. (1997). Population structure and physiological changes within a hot spring microbial mat community following disturbance. "
  298. (1982). Population studies on Artemia in Mono Lake.
  299. (1998). Potential salinity limitations on nitrogen fixation in sediments from
  300. (2003). Production of free and organic iodine by Roseovarills spp. "
  301. (2002). Prokaryotic genetic diversity throughout the salinity gradient of a coastal solar saltern. "
  302. (1998). Proposal of a new halobacterial genus Natrinema gen. nov., with two species Natrinenia pelleritbra nom. nov. and Natrinema pallida nom. nov. "
  303. (2004). Psychroflextis tropicus sp. nov., an obligately halophilic Cytophaga-Flavobacteritim-Bacteroides group bacterium from an Hawaiian hypersaline lake. "
  304. Published only in database (2001)). An extreme halophile in the order Halobacteriales, Noda Institute for Scientific Research,
  305. (1999). published only in NCBI database
  306. (1994). Quantitative cell lysis of indigenous microorganisms and rapid extraction of microbial DNA from sediment. "
  307. (1998). Quantitative measure of small-subunit rRNA gene sequences of the Kingdom Korarchaeota. "
  308. (2000). Radon concentrations in hot spring waters in northern Venezuela. "
  309. (2006). Reclassification of Pseudontonas beijerinckii llof 1935 as Chromohalobacter beijerinckii comb. nov., and emended description of the species. "
  310. (2000). Reclassification of some species of Thiobaciffils to the newly designated genera
  311. (1994). Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms. "
  312. (2002). Recovery of bacteria from glacial and subalacial environments. Department of Microbiology, Columbus, Ohio State University. PhD,
  313. (1997). Recovery of crenarchaeotal ribosomal DNA sequences from freshwater-lake sediments. "
  314. (1994). Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment. "
  315. (1997). Renaut
  316. (2003). Review and re-analysis of domain-specific 16S primers. "
  317. (2006). Review of the African distribution of the brine shrimp genus Artemia. "
  318. (2001). rmdb: the ribosomal RNA operon copy number database. "
  319. (2004). Salegentibacter holothurionim sp. nov., isolated from the edible holothurian Apostichoptis japonicus. "
  320. (2005). Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongy1ocentrolus interineditts. "
  321. (2002). Salinibacter niber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystalliser ponds. "
  322. (1992). Salt lakes in Tnner Mongolia of China.
  323. (1975). Salt-dependent bacteriophage infecting Halobacteritint cutinibrtim
  324. (1984). Separation of chromosomal DNA molecules from yeast by orthogonal-field-alteration gel electrophoresis. "
  325. (1984). Separation of yeast chromosomc-sized DNAs by pulsed field gradient gel electrophoresis. "
  326. (1992). Sequence heterogeneity between the two genes encoding 16S rRNA from the halophilic archaebacterium Haloarcitla marisniorriti. "
  327. (2005). SHI: A novel, spherical halovirus isolated from an Australian hypersaline lake. "
  328. (1980). Sibling species of Arteinia. The Bring Shrimp Artemia.
  329. (1977). Solar Lake (Sinai). 2. Distribution of photosynthetic microorganisms and primary production. "
  330. (1966). Species-diversity and pattem-diversity in the study of ecological succession.
  331. (1998). Specific ribosomal DNA sequences from diverse environmental settings correlate with experimental contaminants. "
  332. (1999). Strand-specific compositional asymmetries in doublestranded DNA viruses. "
  333. (2006). Stratified prokaryote network in the oxic-anoxic transition of a deep-sea halocline. "
  334. (2006). Structure and activity of bacteria] community inhabiting rice roots and the rhizosphere. "
  335. (1977). Structure, growth, and decomposition of laminated algal-bacterial mats in alkaline hot springs. "
  336. (2003). Substrate uptake in extremely halophilic microbial communities revealed by microautoradiography and fluorescence in situ hydridization. "
  337. (2002). Sulfolobits tokodaii sp. nov. (Sutfolobus sp. strain 7), a new member of the genus Sulfolobits isolated from Beppu Hot Springs, Japan. "
  338. (1967). T'he detection of disease clustering and generalized regression approach. "
  339. (2000). Talaeoclimatic indicators of China's Quaternary. "
  340. (2004). Tapping into microbial diversity. "
  341. (2001). Taxonomic characterization of new alkaliphilic and alkalitolerant methanotrophs from soda lakes of the southeastern Transbaikal region and description of Methylomicrobium buryatense sp.
  342. (2004). Taxonomic study of Halonibn1m distribiltun? and proposal of Halonibnim terrestre sp. nov. "
  343. (1994). Taxonomy and Phylogeny of Moderately Halophilic Bacteria. "
  344. (2000). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov.,
  345. (2005). Tbe genome of Salinibacter niber. Convergence and gene exchange among hyperhalophilic bacteria and archaea. "
  346. (1993). Tbermoalkaliphilic lipase-producing Bacillus selected by continuous cultivation. "
  347. (1995). TCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. "
  348. (1997). Tensor CSAMT survey over the sulphur springs thermal area,
  349. (2004). Thage community dynamics in hot springs. "
  350. (1983). The biology of a new gas-vacuolate Cyanobacterium, Daclýylococcopsis salina sp. nov., in Solar Lake "
  351. (2001). The contribution of halophilic Bacteria to the red coloration of saltem crytallizer ponds. "
  352. (2006). The diversity and biogeography of soil bacterial communities. "
  353. (2005). The diversity of culturable organotrophic bacteria from local solar saltems. "
  354. (1994). The ecology of the extremely halophilic archaca. "
  355. (2002). The European database on small subunit ribosomal RNA. "
  356. (1996). The frequency of chimeric molecules as a consequence of PCR co-amplification of 16S rRNA genes from different bacterial species. "
  357. (1993). The haloalkaliphilic archaeon (archaebacterium) Nalronococcits occultits represents a distinct lineage within the Halobacteriales, most closely related to the other haloalkaliphilic lineage (Natronobacterhim). " SYst A12121
  358. (1987). The hydrothermal system of the Calabozos caldera, central Chilean Andes. "
  359. (1996). The largest, highest and lowest lakes of the world: Saline lakes. "
  360. (2002). The long-term effects of UV exclusion on the microbial composition and photosynthetic competence of bacteria in hot-spring microbial mats. "
  361. (2006). The marine viromes of four oceanic regions. "
  362. (1963). The mathematical theory of communication.
  363. (1974). The measurement of species diversity.
  364. (2004). The microbial arsenic cycle in
  365. (2000). The microbial composition of three limnologically disparate hypersaline Antarctic lakes. "
  366. (1999). The nature of the last universal common ancestor. "
  367. (1971). The nonconcept of species diversity: a critique and alternative parameters. "
  368. (1996). The phylogenetic relationship among Ectothiorhodospiraceae: a reevaluation of their taxonomy on the basis of 16S rDNA analyses. "
  369. (1995). The Rehai (Hot Sea) Geothermal System in Tengchong County, Yunnan Province, China. " In Terrestrial Heat Flow and-Geothermal Energy in Asia. Eds:
  370. (1972). The upper temperature limit for eukaryotic organisms. "
  371. (1999). Thennits diversity and strain loss during enrichment. "
  372. (1989). Thennotoga thennanim sp. nov. and Thennotoga neapolitana occurring in African continental solfataric springs. "
  373. (2002). Thermlis rehai sp. nov., isolated from Rehai of Tengchong,
  374. (1985). Thermophilic anaerobic spirochetes in New Zealand hot springs. "
  375. (2002). Thioalkalivibrio thiocyanooxidalls sp. nov. and Thioalkalivibrio paradoxits sp. nov., novel alkaliphilic, obligately autotrophic, sulfuroxidising bacteria from soda lakes able to grow with thiocyanate. "
  376. (2003). Thioalkavibrio nitratireducens sp. nov., a nitrate-reducing member of an autotrophic denitrifying consortium from a soda lake. "
  377. (2005). Thylogenetic characterization of 16S rRNA gene clones from deep-groundwater microorganisms that pass through 0.2-micrometerpore-size filters. "
  378. (2002). Thylogenetic characterization of microbial mats and streamers from a Japanese alkaline hot spring with a thermal gradient. "
  379. (2001). Thylogenetic characterization of the blue filamentous bacterial community from an Icelandic geothen-nal spring. "
  380. (2004). Thylogenetic diversity of Bacteria in the leachate of a full-scale recirculating landfill. "
  381. (1996). Thylogenetic diversity of soda lake alkaliphiles. "
  382. (1998). Thylogenetic evidence for the existence of novel thermophilic bacteria in hot spring sulfur-turf microbial mats in Japan. "
  383. (2002). Thylogenetic structure of the genera Flexibacter. Flexithrix, and Microscilla deduced from 16S rRNA sequence analysis. "
  384. (1977). Thylogenetic structure of the prokaryotic domain: the primary kingdoms. "
  385. (1998). Tindallia magadii gen. nov., sp. nov.: an alkaliphilic anaerobic ammonifier from soda lake deposits. "
  386. (1971). Toward defining the course of evolution: minimum change for a specific tree topology. "
  387. (1990). Towards a natural system of organisms: proposal for the domains Archaea, Bacteria and Eucarya. "
  388. (1998). Transcription and translation in Archaea: a mosaic of eukaryal and bacteria] features. "
  389. (1994). Transcription of the Halophage Phi 11 repressor gene is abolished by transcription from an inversely oriented lytic promoter. "
  390. (2000). Two different and highly organized mechanisms of translation initiation in the archaeon Sulfolobus sotfataricus. "
  391. (1985). Two types of xylanases of alkaliphilic Bacillus sp.
  392. (1992). Uncultivated cyanobacteria, Chloroflexits-like inhabitants, and Spirochete-like inhabitants of a hot spring microbial mat. "
  393. Uncultured haloarchaeon from Zabuye Lake, Tibet China, Chinese Academy of Sciences,
  394. (2006). Unexpected diversity and complexity of the Guerrero Negro hypersaline microbial mat. "
  395. (2001). Unexpected diversity of small eukaryotes in deep-sea Antarctic plankton. "
  396. (2005). UniFrac: a new phylogenctic method for comparing microbial communities. "
  397. (2005). Variability in microbial community and venting chemistry in a sediment-hosted backarc hydrothermal system: Impacts of subseafloor phase-separation. "
  398. (2000). Variations in isotopic compositions of chlorine in evaporation-controlled salt lake brines of Qaidam Basin, China "
  399. (1999). Vertical distribution of methanogens in the anoxic sediment of Rotsee (Switzerland). "
  400. (2005). Vibrio metschnikovii Pneumonia. "
  401. (2005). Viral metagenomics. "
  402. (2000). Virus Taxonomy: Classification and Nomenclature of Viruses. 7h report.
  403. (2001). Viruses from extreme thermal environments. "
  404. (1996). Viruses, plasmids and other genetic elements of thermophilic and hyperthermophilic archaea. "
  405. (2004). Walorubntm tibetense sp. nov., a novel haloalkaliphilic archaeon from Lake Zabuye in Tibet, China. "
  406. (1993). Xylanolytic anaerobic thermophiles from Icelandic hot-springs. "
  407. Yong Study on Kunming low-temperature geothermal field.
  408. (2002). zt: a software tool for simple and partial Mantel tests. "

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