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Use of Vectorette and Subvectorette PCR to Isolate Transgene Flanking DNA

By Maxine J. Allen, Andrew Collick and Alec J. Jeffreys

Abstract

Vectorette PCR permits the specific amplification of DNA segments flanking a known DNA sequence. It enables the application of the PCR where sequence information is only available for one primer site. We now show that vectorette PCR can be used for the systematic mapping and retrieval\ud of transgene flanking DNA. We also show that the sequence of large vectorette PCR fragments can be obtained without cloning, by the production of subvectorette fragments

Publisher: Cold Spring Harbor Laboratory Press.
Year: 1994
DOI identifier: 10.1101/gr.4.2.71
OAI identifier: oai:lra.le.ac.uk:2381/4299
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