Location of Repository

MLH1 mutations differentially affect meiotic functions in Saccharomyces cerevisiae.

By Eva R. Hoffmann, Polina V. Shcherbakova, Thomas A. Kunkel and Rhona H. Borts

Abstract

The full-text of this article is not available on the LRA. The original published version is available from the publisher's website at: http://www.genetics.org/To test whether missense mutations in the cancer susceptibility gene MLH1 adversely affect meiosis, we examined 14 yeast MLH1 mutations for effects on meiotic DNA transactions and gamete viability in the yeast Saccharomyces cerevisiae. Mutations analogous to those associated with hereditary nonpolyposis colorectal cancer (HNPCC) or those that reduce Mlh1p interactions with ATP or DNA all impair replicative mismatch repair as measured by increased mutation rates. However, their effects on meiotic heteroduplex repair, crossing over, chromosome segregation, and gametogenesis vary from complete loss of meiotic functions to no meiotic defect, and mutants defective in one meiotic process are not necessarily defective in others. DNA binding and ATP binding but not ATP hydrolysis are required for meiotic crossing over. The results reveal clear separation of different Mlh1p functions in mitosis and meiosis, and they suggest that some, but not all, MLH1 mutations may be a source of human infertility

Year: 2003
OAI identifier: oai:lra.le.ac.uk:2381/2022
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://hdl.handle.net/2381/202... (external link)
  • http://www.genetics.org/cgi/co... (external link)
  • http://www.genetics.org/ (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.