Skip to main content
Article thumbnail
Location of Repository

Polyphasic characterisation of enrichment cultures from four hypersaline Inner Mongolian Lakes Shangmatala, Ejinnor, Bagaejinnor, and Erliannor.

By Andrew Wallace


Enrichment media were formulated using analysis of the chemistry of the salt lakes Shangmatala, Ejinnor, Bagaejinnor, and Erliannor in Inner Mongolia. These enrichments contained yeast extract with either glycerol, betaine or starch as carbon sources. Samples of salt, brine and mud from the lakes were used as inoculants and enrichments were incubated at 37◦C for 18 days. A total of twenty different bacterial isolates were selected. All isolates were genotypically characterised by their 16S rDNA sequences, thirteen were phenotypically characterised. Despite the unusual carbon sources in the enrichment media analyses revealed that the majority of the isolates were related to organisms previously isolated from similar hypersaline sites. A large number have gene sequence identity to not validly published bacteria. Most isolates were Gram positive bacilli and cocci although there were a small number of Gram negative halomonads. Phenotypically the isolates were different. The majority of isolates could grow over a range of pH and salt concentrations. Isolates from the same lake appear to show similar ability to grow on different culture media. Three isolates significantly degraded starch. All isolates were susceptible to chloramphenicol and to ampicillin. Most isolates have an ribosomal RNA gene sequence identity above 99% to data base entries (lowest identities observed were isolate En1 98% to Bacillus sp. SL5-2 and isolate EJBB2 97% to Bacteroidetes bacterium KMM). It is therefore unlikely that many of the isolates represent new species

Publisher: University of Leicester
Year: 2008
OAI identifier:

Suggested articles


  1. (2001). 16S rDNA sequence analysis of bacterial isolates from biodeteriorated mural paintings in the Servilia tomb. (Necropolis of carmona, doi
  2. (2002). A new phylum of Archaea represented by a nanosized hyperthermophilic symbiont. doi
  3. a novel member of the order Bacillales from a saline soil in China. doi
  4. (2007). A Systematics for Discovering the Fundamental Units doi
  5. (1955). An improved technique for staining red bacteria
  6. (2001). Bacterial Species Determination from DNA-DNA Hybridization by Using Genome Fragments and DNA Microarrays. doi
  7. (1934). Bacteriological studies on the red discoloration of salted hides. doi
  8. (1990). Basic local alignment search tool. doi
  9. (1998). Biology of moderately halophilic aerobic bacteria
  10. (1995). Biotechnological applications and potentialities of halophilic microorganisms. doi
  11. (1993). Chinese salt lakes. doi
  12. (2000). del Moral A doi
  13. Delacroix-Buchet A & Ogier JC (2007). Biodiversity of bacterial ecosystems in traditional Egyptian Domiati cheese. doi
  14. (2005). Diversity of the human intestinal microbial flora.
  15. (2002). Emended descriptions of the genus Micrococcus, Micrococcus luteus 100. BJ1.1G2 101. BJ1.2G1 102. BJ1.2G2 103. BJ1.3G1 104. BJ1.3G2 105.
  16. (2002). Environmental threats to Salt lakes and the likely status of inland saline ecosystems in doi
  17. (2007). Exploitation of Dunaliella for beta-carotene production. doi
  18. (2003). Extremophiles as a source for novel enzymes. doi
  19. (1998). Extremophiles: Microbial Life in Extreme Environments. doi
  20. (2002). Geographical and seasonal distribution of multiple antibiotic resistance of heterotrophic bacteria of Lake Shira.
  21. (2004). Geomicrobiology of high-level nuclear waste-contaminated vadose sediments at the Hanford site, Washington State. doi
  22. (2007). Giordano A & Gambacorta A doi
  23. (2007). Halophilic and Halotolerant Bacteria from River Waters and Shallow Groundwater Along the Rouge River of Southeastern Michigan. doi
  24. (2004). Halotolerant aerobic heterotrophic bacteria from the Great Salt Plains of Oklahoma. doi
  25. (2006). Identification and quantification of archaea involved in primary endodontic infections. doi
  26. (2005). Interhemispheric transport of viable fungi and bacteria from Africa to the Caribbean with soil dust. doi
  27. (2006). Interrelationships between Dunaliella and halophilic prokaryotes in saltern crystallizer ponds doi
  28. (2006). Intracolony variation of bacterial gut microbiota among castes and ages in the fungus-growing termite Macrotermes gilvus doi
  29. (2004). Isolation of haloarchaea that grow at low salinities. doi
  30. (2004). Life at low water activity.Phil.
  31. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 doi
  32. (2004). Methanogenic Archaea and human periodontal disease. doi
  33. (1990). Methanogenic bacteria in human vaginal samples.
  34. (2007). Nanoscale electrical conductivity of the purple membrane monolayer Phys Rev E Stat Nonlin Soft Matter. doi
  35. (1995). Osmoadaptation in bacteria. doi
  36. (2005). Phylogenetic diversity and ecology of environmental doi
  37. (2007). Phylogenetic diversity of gram-positive bacteria cultured from marine sediments. doi
  38. (1999). Phylogenetic relationships of 38 taxa of the genus Staphylococcus based on 16S rRNA gene sequence analysis. doi
  39. (2002). Phylogeny of the family Halomonadaceae based on 23S and 165 rDNA sequence analyses. doi
  40. (2002). Prokaryotic genetic diversity throughout the salinity gradient of a coastal solar saltern doi
  41. (1981). Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria.
  42. reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov doi
  43. (2006). Salicola marasensis gen. nov., sp. nov., an extremely halophilic bacterium isolated from the Maras solar salterns in Peru. doi
  44. (1978). Studies on the Aminopeptidase-Test for the Distinction of Gram-Negative from Gram-Positive Bacteria. doi
  45. (2000). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. doi
  46. (2003). The Desk Encyclopedia of Microbiology. doi
  47. (1986). The ecology and taxonomy of halobacteria. doi
  48. (2003). The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism. doi
  49. (1981). Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin. doi
  50. (2006). Viruses of the Archaea: a unifying view. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.