Nucleobase Protection of Deoxyribo‐ and Ribonucleosides

Abstract

Protecting groups for the imide/lactam function of thymine/uracil and guanine, respectively, prevent irreversible nucleobase modifications that may occur in the presence of alkylating or condensing reagents that are commonly used in nucleoside protection and oligonucleotide synthesis. This unit reviews these protecting groups, and also identifies protecting groups for the exocyclic amino function of cytosine, adenine, and guanine. The unit also explores recent trends in nucleobase protection that permit reliable oligonucleotide synthesis and removal of N‐protecting groups under very mild conditions.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143711/1/cpnc0201.pd