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The effects of neonatal MK-801 treatment on expression of NMDA receptors and NGF in rats

By 贾娇, 李继涛, 黄润虎, 杨红, 苏允爱 and 司天梅

Abstract

目的:探讨新生期重复注射N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体拮抗剂地卓西平马来酸盐(dizocilpine maleate,MK-801)对不同发育阶段大鼠NMDA受体亚基NMDAR1(NR1)、NMDAR2A (NR2A)、NMDAR2B(NR2B)表达及神经生长因子(nerve growth factor,NGF)含量的影响。方法30只新生雄性大鼠随机分为研究组和对照组,各15只。于出生后(postnatal day,PND)5~14d皮下注射MK-801(0.25mg/kg,每日2次)或生理盐水。分别于幼年期(PND15)、青春期(PND42)和成年期(PND70)取脑留取海马、前额叶皮质脑区,用免疫印迹(Western blot)方法检测NR1、NR2A、NR2B表达水平,用ELISA方法测定NGF含量。结果幼年期(PND15)与对照组相比,研究组海马与前额叶NMDA受体各亚基表达水平及NGF含量没有统计学差异(均P>0.05);青春期(PND42)研究组大鼠前额叶NGF含量低于对照组[(56.19±37.87)vs.(152.54±53.92)],差异有统计学意义(P<0.01);成年期(PND70)研究组大鼠海马NR1、NR2A表达水平较对照组增加[NR1:(149.55%±27.00%)vs.(100.00%±32.08%);NR2A:(171.54%±19.85%)vs.(100.00%±51.04%)],差异均有统计学意义(均P<0.05)。结论新生期重复注射MK-801选择性上调成年大鼠海马NMDA受体亚基NR1和NR2A的表达水平,且显著降低青春期大鼠前额叶NGF的含量。提示发育早期阻断NMDA受体对大鼠大脑的神经发育产生远期影响。国家自然科学基金中文核心期刊要目总览(PKU)中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)012733-73

Topics: 地卓西平马来酸盐 N-甲基-D-天冬氨酸受体 神经生长因子 精神分裂症 Dizocilpine maleate N-methyl-D-aspartate receptor Nerve growth factor Schizophrenia
Publisher: 中国神经精神疾病杂志
Year: 2013
DOI identifier: 10.3936/j.issn.1002-0152.2013.12.007
OAI identifier: oai:localhost:20.500.11897/234496
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