Article thumbnail
Location of Repository

Blockade of U50488H on potassium currents of acutely isolated mouse hippocampal CA3 pyramidal neurons

By BD Zou, KF OuYang, YZ Chen, CH Wu and PA Zhou

Abstract

The actions of the opioid agonist U50488H on I-A and I-K were examined in acutely isolated mouse hippocampal CA3 pyramidal neurons using the whole-cell patch clamp technique. U50488H caused a concentration dependent, rapidly developing and reversible inhibition of voltage-activated I-A and I-K. The inhibitory actions were still observed in the presence of 30 muM naloxone or 5 muM nor-binaltorphimine dihydrochloride. The IC50 values for the blockade of I-A and I-K were calculated as 20.1.9 and 3.7 muM, respectively. In the presence of 3.3 muM U50488H, repetitive stimulation induced use-dependent inhibition of I-A and I-K. A 10 muM concentration of U50488H positively shifted the half-activation membrane potential of I-A by +11 mV, but negatively shifted I-K by -14 mV. These results demonstrate that U50488H can directly inhibit neuronal I-A and I-K without involvement of the activation of kappa -opioid receptors. (C) 2001 Elsevier Science B.V. All rights reserved.NeurosciencesSCI(E)PubMed6ARTICLE1-252-5989

Topics: hippocampus, CA3 neuron, patch-clamp, U50488H, potassium current, K+ CURRENTS, CEREBRAL-ISCHEMIA, RAT, AGONIST, DEPOLARIZATION, MECHANISMS, MODULATION, CHANNELS, INVITRO, ANOXIA
Publisher: 脑研究
Year: 2001
DOI identifier: 10.1016/S0006-8993(00)03330-8
OAI identifier: oai:localhost:20.500.11897/200586
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://hdl.handle.net/20.500.1... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.