The Golgi is composed of a stack of cis, medial, trans cisternae that are biochemically distinct.
The stable compartments model postulates that permanent cisternae communicate through
bi-directional vesicles, while the cisternal maturation model postulates that transient
cisternae biochemically mature to ensure anterograde transport. Testing either model
has been constrained by the diffraction limit of light microscopy, as the cisternae are only
10–20 nm thick and closely stacked in mammalian cells. We previously described the
unstacking of Golgi by the ectopic adhesion of Golgi cisternae to mitochondria. Here, we
show that cargo processing and transport continue—even when individual Golgi cisternae are
separated and “land-locked” between mitochondria. With the increased spatial separation of
cisternae, we show using three-dimensional live imaging that cis-Golgi and trans-Golgi remain
stable in their composition and size. Hence, we provide new evidence in support of the stable
compartments model in mammalian cells
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