Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human archived brain samples to demonstrate sensitive, efficient, and unbiased classification of cell types, paving the way for systematic charting of cell atlases. Keywords: Cellular neuroscience; Gene expression; Gene expression analysis; RNA sequencin
To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.