Effect of the exposure to suspended solids on the enzymatic activity in the bivalve Sinonovacula constricta

Abstract

Aquatic animals are susceptible to sudden changes of their living environment but they adopt strategies to cope with adverse environmental challenges. Contamination by suspended solids, often associated with a dramatic change in the concentrations of important water-quality variables is a frequent occurrence in China's coastal waters and estuaries. Here we studied the impact of suspended solids on the activities of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), as well as adenosine triphosphates (including Na+ K+-ATPase, Mg+ +-ATPase, Ca+ +-ATPase) and H+ K+-ATPase in the gills and visceral mass tissues of the molluscan bivalve Sinonovacula constricta exposed (4, 8, 12, 16, 20, and 24 days) to various concentrations of suspended solids. Our results showed that the antioxidant enzymes cooperated closely to effectively scavenge superoxide anion free radicals and H2O2 (which can ultimately inhibit gill activity) through the modification of SOD and/or CAT enzymatic activities. ATPases activity (considered to be a sensitive indicator of toxicity) could play an effective role in the maintenance of functional integrity of the plasma membranes as well as some other intracellular functions. After the exposure, a decrease in the Na+ K+-ATPase, Mg+ +-ATPase, and Ca+ +-ATPase activity of the gills was observed suggesting that they were inhibited by the treatments. These results also indicated that, from day 4 to day 16, exposure to high concentrations of suspended solids had an inhibitory effect on the activity of H+-K+-ATPase in the visceral mass of S. constricta. However, after a period of adaptation the H+-K+-ATPase activity was restored to original levels. Our results suggest that long-term exposure to high levels of suspended solids disturb osmoregulation, gastric acid secretion and digestion, cause oxidative damage, as a consequence of antioxidant enzymes inactivation which eventually damages the gills, affect the food intake and transformation, ultimately resulting in systems failure and eventually death