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Isolation and characterization of ten polymorphic microsatellite loci in Ixodes arboricola, and cross-amplification in three other Ixodes species

By N. van Houtte, A. R. van Oosten, K. Jordaens, E. Matthysen, T. Backeljau and D. J A Heylen

Abstract

We characterized ten polymorphic microsatellite loci from the tree-hole tick, Ixodes arboricola. Loci were screened in 11-18 individuals from three Belgian populations and five to ten alleles were found at each locus. Seven loci did not show deviations from Hardy-Weinberg equilibrium conditions and there were no indications for null alleles at these loci. The three other loci showed significant heterozygote deficiencies in at least one population, and a high potential for the occurrence of null alleles. We observed no effect of potential host DNA on the scoring of the microsatellites. Cross-amplification of the microsatellites was tested in eight specimens of three congeneric species: I. ricinus, I. hexagonus and I. frontalis. Depending on the species, six or seven of the loci were amplified in ≥4 of the 8 specimens and were polymorphic in each of these species (except for Ixaf 11 in I. frontalis and I. ricinus). These loci thus provide a tool for population genetic analysis of I. arboricola. The suitability of these markers needs to be further investigated in its congeners

Topics: Host race formation, Ixodes arboricola, Microsatellite, Tree hole tick, Ecology, Insect Science
Year: 2013
DOI identifier: 10.1007/s10493-013-9702-x
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Provided by: NARCIS
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