Skip to main content
Article thumbnail
Location of Repository

Analysis of corrinoids in ovine tissues

By R J Kelly, Tini M Gruner, J M Furlong and A R Sykes


Corrinoids from various ovine tissue samples (liver, blood, small intestinal fluid and faeces) were analysed using a combination of high-performance liquid chromatography (HPLC) and a radioisotope dilution assay (RIDA) to estimate the distribution of corrinoids - the cobalamins hydroxocobalamin (OH-cbl), methylcobalamin (me-cbl) and 5-deoxyadenosylcobalamin (ado-cbl), and cobalamin analogues - in these tissues. Samples were taken from either cobalt-deficient or cobalt-replete ewes, and ruminant and pre-ruminant lambs. In liver, ado-cbl predominated, followed by analogues, OH-cbl and me-cbl. Supplementation with either cobalt (ruminant) or vitamin B12 injections (pre-ruminant) increased the amount of ado-cbl and decreased analogues. In blood, OH-cbl predominated, followed by ado-cbl, analogues and me-cbl, respectively. In small intestinal fluid, the distribution from largest to smallest percentage was analogues, ado-cbl, OH-cbl and me-cbl. In faeces, analogues constituted the greatest proportion, followed by OH-cbl, ado-cbl and me-cbl, respectively. Owing to the small sample sizes only cautionary interpretations can be made. In contrast to humans, where me-cbl constitutes the highest proportion of corrinoids in plasma and ado-cbl in the liver, in sheep the amount of ado-cbl was consistently higher than me-cbl in all tissues. This may be due to the higher metabolic need of sheep for ado-cbl due to gluconeogenesis. Analogues and OH-cbl were found in each tissue, contrary to previous postulations. The much higher amount of vitamin B12 in small intestinal fluid compared with faeces indicates that a large proportion of the vitamin is absorbed by the gastro-intestinal tract

Topics: cobalamins, hydroxocobalamin, methylcobalamin, 5-deoxyadenosylcobalmin, HPLC, radioisotope dilution assay, Alternative and Complementary Medicine, Medicine and Health Sciences, Nursing
Publisher: ePublications@SCU
Year: 2006
DOI identifier: 10.1002/bmc.604
OAI identifier:
Provided by: ePublications@SCU
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • (external link)
  • (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.