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A rapid and sensitive bioassay to measure bone morphogenetic protein activity-1

By Lior Zilberberg (82258), Peter ten Dijke (82259), Lynn Y Sakai (60639) and Daniel B Rifkin (82260)


<p><b>Copyright information:</b></p><p>Taken from "A rapid and sensitive bioassay to measure bone morphogenetic protein activity"</p><p></p><p>BMC Cell Biology 2007;8():41-41.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2094707.</p><p></p>ell and 5 × 10cells/well, respectively. The reporter cells were incubated with increasing concentrations of the indicated recombinant BMPs in DMEM, 0.1% BSA. After 15 h incubation, BMP activity was assessed by measuring luciferase activity in the cell lysates. (A) BMP-2. (B) BMP-4. (C) BMP-6. (D) BMP-7. Background luciferase level in the control sample (no BMP) was subtracted from each of the experimental values. The results are expressed as relative luciferase units (RLU). Each point represents the mean ± SEM of triplicate wells from 1 representative experiment. Similar results were obtained in two separate experiments

Topics: Genetics, rapid, sensitive, bioassay, measure, bone, morphogenetic, protein
Year: 2011
DOI identifier: 10.6084/m9.figshare.65836.v1
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Provided by: FigShare
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