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Differential effects of energy stress on AMPK phosphorylation and apoptosis in experimental brain tumor and normal brain-1

By Purna Mukherjee (83893), Tiernan J Mulrooney (83894), Jeremy Marsh (83895), Derek Blair (83896), Thomas C Chiles (83897) and Thomas N Seyfried (79457)

Abstract

4 hours. a) Cell lysates were probed with specific phosphorylated and non-phosphorylated antibodies for western blot as indicated. Dose dependent increase in AMPK phosphorylation was evident in CT-2A and astrocytes but caspase-3 and PARP cleavage was noted only in AICAR treated CT-2A cells. b) Cells were treated with 1.0 mM AICAR in glucose, glutamine and serum containing media for 24 hours and treated with Annexin V and PI stain as described in methods. Labeled cells were analyzed by flow cytometry. 8–10% apoptotic cells were noted in CT-2A cells treated with AICAR compared to untreated cells. No significant cell-death was noticed in treated astrocytes. All experiments were done in triplicate.<p><b>Copyright information:</b></p><p>Taken from "Differential effects of energy stress on AMPK phosphorylation and apoptosis in experimental brain tumor and normal brain"</p><p>http://www.molecular-cancer.com/content/7/1/37</p><p>Molecular Cancer 2008;7():37-37.</p><p>Published online 12 May 2008</p><p>PMCID:PMC2397440.</p><p></p

Topics: Neuroscience, differential, tumor, brain, experimental, apoptosis, phosphorylation, ampk, stress, energy, effects, normal
Year: 2011
DOI identifier: 10.6084/m9.figshare.68084.v1
OAI identifier: oai:figshare.com:article/68084
Provided by: FigShare
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