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Representative images of U-2 OS cells treated with 100 nM epothilone B or pretubulysin for 48 h.

By Jennifer Herrmann (152429), Yasser A. Elnakady (164316), Romina M. Wiedmann (164319), Angelika Ullrich (164321), Manfred Rohde (61075), Uli Kazmaier (164323), Angelika M. Vollmar (164324) and Rolf Müller (11963)

Abstract

<p>Nuclei were stained with Hoechst 33342 (pseudocolor blue, left panel), and γH2A.X was visualized by immunofluorescence (pseudocolor red, right panel). Arrows indicate fragmented nuclei with bright fluorescent spots due to H2A.X phosphorylation events. Images were acquired on a BD Pathway 855 automated microscope and subsequently processed and analyzed in AttoVision v1.6.2 (the full HCS analysis is provided as supplementary material, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037416#pone.0037416.s002" target="_blank">Figure S2</a>).</p

Topics: Biochemistry, Cell Biology, Molecular Biology, Chemistry, Cancer, images, u-2, os, cells, treated, 100, nm, epothilone, pretubulysin, 48
Year: 2012
DOI identifier: 10.1371/journal.pone.0037416.g010
OAI identifier: oai:figshare.com:article/308027
Provided by: FigShare
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