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Multiple input signals, along with protein and RNA elements, regulate the <i>eut</i> locus in <i>Enterococcus faecalis</i>.

By Arati Ramesh (159304), Sruti DebRoy (159313), Jonathan R. Goodson (159318), Kristina A. Fox (159324), Herbey Faz (159333), Danielle A. Garsin (159338) and Wade C. Winkler (159342)

Abstract

<p>A) The organization of the <i>E. faecalis eut</i> locus is shown schematically. The EutV/EutW two component regulatory system responds to ethanolamine to stimulate EutW autophosphorylation followed by phosphoryl transfer to EutV <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002666#pgen.1002666-DelPapa1" target="_blank">[15]</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002666#pgen.1002666-Fox1" target="_blank">[17]</a>. Phosphorylated EutV is hypothesized to prevent formation of four different intrinsic terminator sites (red) within the <i>eut</i> pathway <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002666#pgen.1002666-Fox1" target="_blank">[17]</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002666#pgen.1002666-Baker1" target="_blank">[18]</a>. Additionally, an AdoCbl-sensing riboswitch is located upstream of <i>eutG </i><a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002666#pgen.1002666-Galperin1" target="_blank">[14]</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002666#pgen.1002666-Garsin1" target="_blank">[16]</a>. B) Expression of <i>lacZ</i> translational fusions to the <i>eutP</i> 5′ leader region is shown as bar graphs and is described in the text. Each fusion is represented by a color with the darker shade indicating the wild type background and the lighter shade designating the <i>eutVW</i> background. Presence of both AdoCbl and ethanolamine was required for induction of <i>eutP</i> containing the wild-type leader sequence (blue). Deletion of the EutV/EutW two-component regulatory system abolished <i>eutP</i> induction (light blue). Deletion of the terminator in the leader, <i>eutP</i>ΔT abolished EutV/EutW dependency (red/light red). The vector control in both backgrounds is shown in black/gray. (C) Expression of <i>lacZ</i> translational fusions to the <i>eutS</i> 5′ leader region. Color scheme is described in the figure.</p

Topics: Biochemistry, Microbiology, Genetics, rna, locus
Year: 2012
DOI identifier: 10.1371/journal.pgen.1002666.g002
OAI identifier: oai:figshare.com:article/296669
Provided by: FigShare
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