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Free Radical Scavenging Activity of 4‑(3,4-Dihydroxybenzoyloxymethyl)phenyl‑<i>O</i>‑β‑d‑glucopyranoside from Origanum vulgare and Its Protection against Oxidative Damage

By Chia-Hua Liang (2056492), Leong-Perng Chan (2056501), Hsiou-Yu Ding (2056495), Edmund Cheung So (2056486), Rong-Jyh Lin (448037), Hui-Min Wang (118099), Ying-Ging Chen (2056489) and Tzung-Han Chou (2056498)

Abstract

4-(3,4-Dihydroxybenzoyloxymethyl)­phenyl-<i>O</i>-β-d-glucopyranoside (DBPG), a polyphenolic glycoside, isolated from Origanum vulgare has shown 1,1-diphenyl-2-picrylhydrazyl (DPPH<sup>•</sup>)-scavenging capacity in previous work. This study demonstrated that DBPG exhibits antioxidant activity by a series of DPPH<sup>•</sup>, 2,2′-azinobis­(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS<sup>•+</sup>), and superoxide anion radical (O<sub>2</sub><sup>•–</sup>) radical-scavenging assays. The inhibition of lipid peroxidation (LP) by DBPG exceeded that by l-ascorbic acid (AA) in a liposome model system. Adding DBPG to mouse liver and brain tissue inhibited the formation of thiobarbituric acid reactive substances (TBARS) to a greater extent than did trolox. In the oxygen stress test, BNLCL2 and HaCaT cells pretreated with DBPG showed increased activities of glutathione peroxidase (GPx), perhaps as a result of reduction of the production of reactive oxygen species (ROS). These findings proved that DBPG had antioxidant activities and a cytoprotective effect in hepatocytes and keratinocytes, suggesting that DBPG may be a useful food and cosmetic additive

Topics: Biochemistry, Medicine, Cell Biology, Neuroscience, Physiology, Biotechnology, Cancer, Chemical Sciences not elsewhere classified, TBARS, AA, liposome model system, BNLCL, ROS, oxygen stress test, reactive oxygen species, Origanum vulgare, HaCaT cells pretreated, ABTS, DBPG exhibits antioxidant activity, thiobarbituric acid reactive substances, LP, DPPH, Free Radical Scavenging Activity
Year: 2012
DOI identifier: 10.1021/jf302329m.s001
OAI identifier: oai:figshare.com:article/2497987
Provided by: FigShare
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