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Synthesis and characterization of ganglioside bead array.

By Atul Asati (139018), Olga Kachurina (139020) and Anatoly Kachurin (139022)

Abstract

<p>A scheme of conjugation of various gangliosides on Bioplex beads via modified EDC/NHS chemistry (<b>a</b>). Dilution-dependent fluorescent response displayed by ganglioside conjugated beads testing various anti-ganglioside rabbit sera (<b>b</b>). Coupling of gangliosides to beads occurred via secondary amine next to ceramide moiety (<b>c</b>). Ceramidase and lipase treatment of beads negate the reporter fluorescent signal (<b>d</b>). The radical R circled in dashed red signifies the presence of sialic acid groups (one in GK1 and GM2, two in GD1b and none in GA1).</p

Topics: Biophysics, Biochemistry, Neuroscience, Chemistry, Immunology, characterization, ganglioside, bead
Year: 2012
DOI identifier: 10.1371/journal.pone.0042681.g001
OAI identifier: oai:figshare.com:article/253549
Provided by: FigShare
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