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The development of the human oncogenic EGFR induced Muv model.

By Young-Ki Bae (137892), Jee Young Sung (137897), Yong-Nyun Kim (137903), Sunshin Kim (137908), Kyeong Man Hong (137913), Heung Tae Kim (137917), Min Sung Choi (137920), Jae Young Kwon (137924) and Jaegal Shim (137928)


<p>(A) LET-23 and LET-23-based chimeric receptor constructs. All constructs were designed to express each chimeric receptor from the <i>let-23</i> promoter. (B) The LET-23::hEGFR-TK chimera rescues the vulvaless phenotype of the <i>let-23(sy1)</i> mutant. (C) A comparison of several Muv mutants and the <i>jgIs6</i> transgenic strain which expresses LET-23::hEGFR-TK[L858R]. <i>C. elegans</i> expressing LET-23::hEGFR-TK[L858R] exhibited a larger pseudovulva compared to <i>let-23(sa62)</i> and <i>let-60(n1700)</i> Muv mutants. (D) Hoechst33342 (H33342) staining of the pseudovulval region in <i>jgIs6</i> revealed many nuclei. The boxed region of the lower panel is enlarged in the right panel. (E) Expression of a 1° cell marker, CDH-3::GFP in the wild-type worm and <i>jgIs6</i>. CDH-3::GFP is highly expressed both in the vulva and pseudovulva of <i>jgIs6</i>. (F) Expression of 2° vulval cell fate markers in the <i>lin-15</i> Muv mutant and <i>jgIs6</i>. Reporter genes controlled by promoters of <i>egl-17</i> and <i>dhs-31</i> were expressed in the vulva and pseudovulva. Arrowheads indicate normal vulvae and small arrows indicate pseudovulvae. Scale bars, 50 µm.</p

Topics: Physiology, Biotechnology, Cancer, oncogenic, egfr, induced, muv
Year: 2012
DOI identifier: 10.1371/journal.pone.0042441.g001
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Provided by: FigShare
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