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HGF induces cells migration and MMP-2 up-regulation through NF-κB.

By Hsi-Kai Tsou (104489), Hsien-Te Chen (104492), Ya-Huey Hung (104494), Chia-Hao Chang (104496), Te-Mao Li (104498), Yi-Chin Fong (104502) and Chih-Hsin Tang (104506)


<p>(A, B, and E) Cells were pretreated for 30 min with PDTC (10 µM), TPCK (3 µM), or curcumin (3 µM) or transfected with dominant-negative (DN) mutants of IKKα or IKKβ for 24 h, followed by stimulation with HGF. Cell migration and MMP-2 expression was examined by Transwell, qPCR, and zymography assays. (C and D) JJ012 cells were transfected with c-Jun or SP-1 siRNA followed by stimulation with HGF; cell migration and MMP-2 expression was examined by Transwell and qPCR assays. (F) JJ012 cells were incubated with HGF for the indicated time intervals, and p-IKK, p-IκB, and p-p65 expression was determined by western blotting. Results are expressed as mean ± S.E. *<i>p</i><0.05 compared with control; #<i>p</i><0.05 compared with HGF-treated group.</p

Topics: Cell Biology, Physiology, Cancer, induces, cells, mmp-2, up-regulation
Year: 2013
DOI identifier: 10.1371/journal.pone.0053974.g006
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Provided by: FigShare
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