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BRG1 promotes hepatocarcinogenesis by regulating proliferation and invasiveness

By Benedikt Kaufmann (4243171), Baocai Wang (3559367), Suyang Zhong (4243174), Melanie Laschinger (374691), Pranali Patil (4243168), Miao Lu (2657470), Volker Assfalg (3559370), Zhangjun Cheng (3559355), Helmut Friess (10425), Norbert Hüser (287584), Guido von Figura (229502) and Daniel Hartmann (229506)

Abstract

<div><p>The chromatin remodeler complex SWI/SNF plays an important role in physiological and pathological processes. Brahma related gene 1(BRG1), a catalytic subunit of the SWI/SNF complex, is known to be mutated in hepatocellular carcinoma (HCC). However, its role in HCC remains unclear. Here, we investigate the role of BRG1 on cell growth and invasiveness as well as its effect on the expression of putative target genes. Expression of BRG1 was examined in human liver tissue samples and in HCC cell lines. In addition, BRG1 was silenced in human HCC cell lines to analyse cell growth and invasiveness by growth curves, colony formation assay, invasion assay and the expression of putative target genes. BRG1 was found to be significantly increased in HCC samples compared to non-HCC samples. In addition, a declined proliferation rate of BRG1-silenced human HCC cell lines was associated with a decrease of expression of cyclin family members. In line with a decreased invasiveness of BRG1-siRNA-treated human HCC cell lines, down-regulation of MMP7 was detected. These results support the hypothesis that overexpression of BRG1 increases cell growth and invasiveness in HCC. Furthermore, the data highlight cyclin B, E and MMP7 to be associated with BRG1 during hepatocarcinogenesis.</p></div

Topics: Biochemistry, Microbiology, Genetics, Molecular Biology, Cancer, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, SWI, cyclin family members, colony formation assay, analyse cell growth, invasivenes, BRG 1 increases cell growth, target genes, liver tissue samples, BRG 1, MMP 7, HCC cell lines
Year: 2017
DOI identifier: 10.1371/journal.pone.0180225
OAI identifier: oai:figshare.com:article/5199454
Provided by: FigShare
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