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Karakterizacija β-glukana izoliranih iz pivskoga kvasca te osušenih različitim metodama

By Vesna Zechner-Krpan, Vlatka Petravić-Tominac, Iva Gospodarić, Lana Sajli, Senka Đaković and Jelena Filipović-Grčić

Abstract

Two different procedures have been used for isolation of water-insoluble ß-glucans from brewer’s yeast: alkaline-acidic isolation (AA) and alkaline-acidic isolation with mannoprotein removal (AAM). The obtained ß-glucans were then dried by air-drying, lyophilization and combination of sonication and spray-drying. ß-Glucan preparations obtained by AA and AAM isolations had similar values of dry mass, total polysaccharides, proteins and organic elemental microanalysis. The mass fractions of ß-glucan in total polysaccharides were significantly affected by different isolation procedures. Fourier transform infrared (FTIR) spectra of all preparations had the appearance typical for (1→3)-ß-D-glucan. Lyophilization and especially air-drying caused a higher degree of agglomeration and changes in ß-glucan microstructure. Sonication followed by spray-drying resulted in minimal structural changes and negligible formation of agglomerates.Netopljivi β-glukani izolirani su iz pivskoga kvasca primjenom dvaju različitih postupaka: alkalno-kiselinskog (AK) i alkalno-kiselinskog s uklanjanjem manoproteina (AKM). Dobiveni pripravci β-glukana osušeni su na zraku, liofilizacijom i raspršivanjem uz prethodnu obradu ultrazvukom. Takvi su pripravci, dobiveni AK i AKM postupcima, imali približno iste vrijednosti suhe tvari, ukupnih polisaharida i proteina, te udjele ugljika, dušika i kisika određene organskom mikroanalizom. Na udio β-glukana u ukupnim polisaharidima bitno su utjecali različiti postupci izolacije. FT-IR spektri svih pripravaka imali su izgled tipičan za (1→3)-β-D-glukan. Sušenjem na zraku i liofilizacijom došlo je do značajnih promjena strukture čestica i aglomeracije ß-glukana. Sušenje raspršivanjem uz prethodnu obradu ultrazvukom nije dovelo do stvaranja nakupina čestica kao ni do znatnih promjena njihove strukture

Topics: ß-glucan; ß-glucan isolation; brewer’s yeast; Saccharomyces cerevisiae, β-glukan; izolacija β-glukana; pivski kvasac; Saccharomyces cerevisiae
Publisher: Faculty of Food Technology and Biotechnology, University of Zagreb
Year: 2010
OAI identifier: oai:hrcak.srce.hr:53628

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