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Priprava uzoraka i određivanje diuretika u tjelesnim tekućinama čovjeka pomoću RPHPLC

By DRAGICA ZENDELOVSKA and TRAJČE STAFILOV

Abstract

This article described reverse phase high-performance liquid chromatography (RPHPLC) methods for determination of diuretics in different human body fluids (whole blood, plasma, serum or urine). Sample preparation procedures including solid-phase extraction, liquid-liquid extraction, dilution, precipitation as well as automated RPHPLC procedures were discussed in order to give advantages and disadvantages of each type of sample preparation. Also, values for analytical recovery of each procedure used for sample preparation are summarized. The most important RPHPLC parameters (detection mode, stationary phase, mobile phase, sensitivity, etc.) are also summarized and discussed.U članku se opisuje metoda određivanja diuretika u različitim tjelesnim tekućinama čovjeka (punoj krvi, plazmi, serumu ili urinu) inverzno-faznom visokotlačnom tekućinskom kromatografijom (RPHPLC). Priprava uzoraka uključuje postupke čvrsto-tekuće ili tekuće-tekuće ekstrakcije, razrijeđivanje, taloženje ili automatizirani RPHPLC postupak. Diskutiraju se prednosti i nedostaci različitih priprava uzoraka. Sažete su vrijednosti za analitički povrat svakog pojedinog postupka. Također se sumiraju i diskutiraju najvažniji RPHPLC parametri (način detekcije, stacionarna faza, mobilna faza, osjetljivost, itd.)

Topics: diuretics; RPHPLC; determination; human body fluids, diuretici; RPHPLC, određivanje tjelesne tekućine
Publisher: Croatian Pharmaceutical Society
Year: 2006
OAI identifier: oai:hrcak.srce.hr:4548

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