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Alkaline protease production by solid state fermentation on polyurethane foam

By Chen Hongzhang, Wang Hui, Zhang Aijun and Li Zuohu

Abstract

This paper investigated the process of solid state fermentation (SSF) using PUF (polyurethane foam) as inert solid support to produce alkaline protease. Maximal enzyme activity was 2185U/ml at pH 9.0, incubation temperature 32 0C inoculum amount of 1.0 % (v/v) , nutrient solution3.75 ml/g PUF, incubation time for 2 h and 15.0 mM of added CaCl2. Under the same conditions, the yield of alkaline protease produced by SSF using PUF as support is higher than that by submerged fermentation (SMF)

Topics: Inert support; Solid state fermentation; Alkaline protease; PUF 
Publisher: Croatian Society of Chemical Engineers
Year: 2006
OAI identifier: oai:hrcak.srce.hr:3497

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