Floristic research on the island of Biševo was carried out during the spring of 2000 and 2001. A total of 32 taxa of vascular plants were recorded for the first time. Among them, 23 taxa are autochthonous and 9 are cultivated or formerly cultivated. The island is new locality for orchids Ophrys tommasinii Vis. and Ophrys x lyrata H. Fleischm. This paper also confirms the existence of the neglected species Reichardia intermedia (Sch.Bip.) Cout. and until now doubtful species Euphorbia terracina L. in the Croatian flora. On the basis of our research and the floristic data published so far, flora of the island of Biševo now consists of 421 plant taxa, which were recorded before the fire that occurred in August of 2003, when 80% of the island was devastated
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