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Surface imprinted beads for the recognition of human serum albumin

By Francesca Bonini, Sergey A. Piletsky, Anthony P. F. Turner, Adolfo Speghini and Alessandra Bossi

Abstract

The synthesis of poly-aminophenylboronic acid (ABPA) imprinted beads for the recognition of the protein human serum albumin (HSA) is reported. In order to create homogeneous recognition sites, covalent immobilisation of the template HSA was exploited. The resulting imprinted beads were selective for HSA. The indirect imprinting factor (IF) calculated from supernatant was 1.6 and the direct IF, evaluated from the protein recovered from the beads, was 1.9. The binding capacity was 1.4 mg/g, which is comparable to commercially available affinity materials. The specificity of the HSA recognition was evaluated with competitive experiments, indicating a molar ratio 4.5/1 of competitor was necessary to displace half of the bound HSA. The recognition and binding of the imprinted beads was also tested with a complex sample, human serum and targeted removal of HSA without a loss of the other protein components was demonstrated. The easy preparation protocol of derivatised beads and a good protein recognition properties make the approach an attractive solution to analytical and bio-analytical problems in the field of biotechnology

Topics: Molecularly imprinted polymers, Human serum albumin, 3-Aminophenylboronic acid, Serum depletion, Proteome
Publisher: Elsevier Science B.V., Amsterdam.
Year: 2007
DOI identifier: 10.1016/j.bios.2006.12.034
OAI identifier: oai:dspace.lib.cranfield.ac.uk:1826/3431
Provided by: Cranfield CERES
Journal:

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