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Differentiation and genetic analysis of Rhodnius prolixus and Rhodnius colombiensis by rDNA and RAPD amplification

By Carlos Jaramillo, María Fernanda Montaña, Lyda Raquel Castro, Gustavo A Vallejo and Felipe Guhl

Abstract

Domiciliated Rhodnius prolixus and sylvatic R. colombiensis were analyzed in order to confirm their genetic divergence and verify the risk that the latter represents in the domiciliation process, and to provide tools for identifying the sources of possible reinfestation by triatomines in human dwellings allowing control programs to be undertaken. Comparison of random amplified polymorphic DNA amplification patterns and cluster analysis suggests reproductive discontinuity between the two species. The calculated statistical F value of 0.24 and effective migration rate of 0.6 individuals per generation are insufficient to maintain genetic homogeneity between them and confirm the absence of present genetic flow. R. colombiensis presents higher intrapopulation variability. Polymerase chain reaction of ribosomal DNA supports these findings. The low genetic flow between the two species implies that R. colombiensis do not represent an epidemiological risk for the domiciliary transmission of Trypanosoma cruzi in the Tolima Department. The lower variability of the domiciliated R. prolixus could result in greater susceptibility to the use of pesticides in control programs

Topics: Rhodnius prolixus, Rhodnius colombiensis, domiciliated and sylvatic triatomines, molecular markers, random amplified polymorphic DNA, ribosomal genes, control programs, Microbiology, QR1-502, Science, Q, DOAJ:Microbiology, DOAJ:Biology, DOAJ:Biology and Life Sciences, Arctic medicine. Tropical medicine, RC955-962, Special situations and conditions, RC952-1245, Internal medicine, RC31-1245, Medicine, R, DOAJ:Internal medicine, DOAJ:Medicine (General), DOAJ:Health Sciences
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Year: 2001
DOI identifier: 10.1590/S0074-02762001000800003
OAI identifier: oai:doaj.org/article:81d85661f6254774b4da7aaa01a7301e
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