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Optimization in Detecting Multiply-charged Protein Ions using MALDI TOF-MS

By Jihyeon Lee, Jangmi Hong, Taehee Kim and Jeongkwon Kim


The effects of trifluoroacetic acid (TFA) were evaluated on the generation of multiply charged ions of cytochrome c in a 2-nitrophloroglucinol (2-NPG) matrix in high-vacuum, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The presence of 1% TFA in the 2-NPG matrix solution was more effective in generating multiply charged protein ions than matrix solutions containing 0.1% or 0% TFA. Regarding the matrix itself, with 1% TFA, 2-NPG was significantly more effective in generating multiply charged ions than 2,5-dihydroxybenzoic acid (2,5-DHB). The maximum charge state of cytochrome c was +8 when using a 2-NPG matrix containing 1% TFA

Topics: MALDI-TOF MS, 2-nitrophloroglucinol, Multiply charged ions, Laser spray ionization, Analytical chemistry, QD71-142, Chemistry, QD1-999, Science, Q, DOAJ:Analytical Chemistry, DOAJ:Chemistry
Publisher: Korean Society for Mass Spectrometry
Year: 2013
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