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Cloning and characterization of a glucosyltransferase from <it>Crocus sativus </it>stigmas involved in flavonoid glucosylation

By Ahrazem Oussama, Mozos Almudena, Moraga Ángela and Gómez-Gómez Lourdes

Abstract

<p>Abstract</p> <p>Background</p> <p>Flavonol glucosides constitute the second group of secondary metabolites that accumulate in <it>Crocus sativus </it>stigmas. To date there are no reports of functionally characterized flavonoid glucosyltransferases in <it>C. sativus</it>, despite the importance of these compounds as antioxidant agents. Moreover, their bitter taste makes them excellent candidates for consideration as potential organoleptic agents of saffron spice, the dry stigmas of <it>C. sativus</it>.</p> <p>Results</p> <p>Using degenerate primers designed to match the plant secondary product glucosyltransferase (PSPG) box we cloned a full length cDNA encoding CsGT45 from <it>C. sativus </it>stigmas. This protein showed homology with flavonoid glucosyltransferases. <it>In vitro </it>reactions showed that CsGT45 catalyses the transfer of glucose from UDP_glucose to kaempferol and quercetin. Kaempferol is the unique flavonol present in <it>C. sativus </it>stigmas and the levels of its glucosides changed during stigma development, and these changes, are correlated with the expression levels of CsGT45 during these developmental stages.</p> <p>Conclusion</p> <p>Findings presented here suggest that CsGT45 is an active enzyme that plays a role in the formation of flavonoid glucosides in <it>C. sativus</it>.</p

Topics: Botany, QK1-989, Science, Q, DOAJ:Botany, DOAJ:Biology, DOAJ:Biology and Life Sciences
Publisher: BioMed Central
Year: 2009
DOI identifier: 10.1186/1471-2229-9-109
OAI identifier: oai:doaj.org/article:1630c0f1b4964083ae4a1ebe3c99530b
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